Evidence that the rapid binding of newly accumulated noradrenaline within synaptosomes involves synaptic vesicles

Résultat de recherche: Articleexamen par les pairs

10 Citations (Scopus)

Résumé

When rat brain synaptosomes were incubated with [3H]noradrenaline for 1 min and then exposed to osmotic shock, only about 20% of the newly accumulated [3H]noradrenaline was released. It would appear that most, but possibly not all of the newly accumulated [3H]noradrenaline is rapidly bound to some particulate cytoplasmic constituent within the synaptosome. [3H]Dopamine and [3H]5-hydroxytryptamine were also rapidly bound within synaptosomes but [3H]glycine and [3H]γ-aminobutyric acid were not. Reserpinization (5 mg/kg, i.p., 24 h before preparation) only slightly reduced the initial rate of [3H]noradrenaline uptake by synaptosomes. However, when reserpinized synaptosomes were osmotically shocked, most of the newly accumulated radioactivity was released; this radioactivity was identified chromatographically as [3H]noradrenaline. On the basis of the findings with reserpinized preparations, it seems likely that (1) the rapid intrasynaptosomal binding involves synaptic vesicles and (2) the neuronal membrane transport system itself may be capable of driving the uptake of noradrenaline by nerve-terminals. The rapid vesicular binding observed may not be essential for the accumulation of the amine by presynaptic terminals during brief exposures.

Langue d'origineEnglish
Pages (de-à)87-96
Nombre de pages10
JournalBrain Research
Volume108
Numéro de publication1
DOI
Statut de publicationPublished - mai 21 1976

Note bibliographique

Funding Information:
The author thanks Diana Webb for her expert technical assistance. This work was supported by a grant from the Medical Research Council of Canada.

ASJC Scopus Subject Areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

PubMed: MeSH publication types

  • Journal Article

Empreinte numérique

Plonger dans les sujets de recherche 'Evidence that the rapid binding of newly accumulated noradrenaline within synaptosomes involves synaptic vesicles'. Ensemble, ils forment une empreinte numérique unique.

Citer