Résumé
Aim To explore the spatial and temporal expression patterns of DAB1 and Reelin in the developing and postnatal healthy human kidneys as potential determinants of kidney development. Methods Paraffin-embedded fetal kidney tissue between the 13/14th and 38th developmental weeks (dw) and postnatal tissue at 1.5 and 7 years were stained with DAB1 and Reelin antibodies by double immunofluorescence. Results During the fetal kidney development and postnatal period, DAB1 and Reelin showed specific spatial expression pattern and diverse fluorescence intensity. During the fetal period, DAB1 was strongly expressed in the distal convoluted tubules (DCT), with strong reactivity, and diversely in the proximal convoluted tubules (PCT) and glomeruli. In the postnatal period, DAB1 expression decreased. The strongest Reelin expression in early fetal stages was observed in the PCT. In the postnatal period, Reelin expression decreased dramatically in all observed structures. These two markers were colocalized during early developmental stages, mostly in PCT, DCT, and podocytes. Conclusion The appearance of DAB1 and Reelin during fetal kidney development confirms their potential significant role in the formation of kidney structure or function. High DAB1 expression in the DCT implies its regulatory role in tubular formation or function maintenance during development. Reelin was highly expressed in human kidneys at early fetal stages, mostly in the PCT, while at later fetal stages and postnatal period its expression decreased.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 521-531 |
| Nombre de pages | 11 |
| Journal | Croatian Medical Journal |
| Volume | 60 |
| Numéro de publication | 6 |
| DOI | |
| Statut de publication | Published - déc. 1 2019 |
Note bibliographique
Funding Information:Funding This work was supported by the Croatian Science Foundation (Grant No. IP-06-2016-2575). ethical approval given by Ethics Committee of University Hospital Center Split (class: 003-08/16-03/0001, approval number: 2181-198-03-04-16-0024). Declaration of authorship AR, MGD, MS, VŠ, KW, YK, and KV conceived and designed the study; AR, MJ, IŠ, IK, IR, and VL acquired the data; AR, NF, NK, SZT, VM, JP, SK, BK, MSB, and KV analyzed and interpreted the data; AR, MJ, IŠ, IK, IR, VL, and KW drafted the manuscript; NF, MGD, NK, SZT, MS, VŠ, VM, JP, SK, BK, YK, MSB, and KV critically revised the manuscript for important intellectual content; all authors gave approval of the version to be submitted; all authors agree to be accountable for all aspects of the work. Competing interests KV is a member of the Editorial Board of the Croatian Medical Journal. To ensure that any possible conflict of interest relevant to the journal has been addressed, this article was reviewed according to best practice guidelines of international editorial organizations. All authors have completed the Unified Competing Interest form at www.icmje.org/coi_disclosure.pdf (available on request from the corresponding author) and declare: no support from any organization for the submitted work; no financial relationships with any organizations that might have an interest in the submitted work in the previous 3 years; no other relationships or activities that could appear to have influenced the submitted work.
Publisher Copyright:
© 2019 Medicinska Naklada Zagreb. All rights reserved.
ASJC Scopus Subject Areas
- General Medicine
ODD de l’ONU
Cette action contribue à la réalisation des objectifs de développement durable suivants
