Gene rIII is the nearest downstream neighbour of bacteriophage T4 gene 31

Aušra Raudonikiene, Rimas Nivinskas

Résultat de recherche: Articleexamen par les pairs

7 Citations (Scopus)

Résumé

The nucleotide sequence of the 2218-bp T4 DNA fragment encompassing gene 31 and five complete open reading frames (ORFs) is presented. We show here that one of these ORFs, ORF31.-1, located downstream from gene 31, is the rIII gene. The position of the gene was established by comparison with the sequences of the rIII gene mutants, r67, rES40 and rBB9. The ORF corresponding to the rIII gene encodes a basic protein of 82 amino acids with an Mr of 9323 and a pI of 9.28. According to the Chou and Fasman [Adv. Enzymol. 47 (1978) 45-148] secondary structure prediction, the rIII protein has a relatively high helical content. In addition, discrepancies with the overlapping sequences determined by other authors in this region are indicated.

Langue d'origineEnglish
Pages (de-à)85-90
Nombre de pages6
JournalGene
Volume114
Numéro de publication1
DOI
Statut de publicationPublished - mai 1 1992
Publié à l'externeOui

ASJC Scopus Subject Areas

  • Genetics

PubMed: MeSH publication types

  • Journal Article

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