Résumé
Halide permeability sequences were obtained from reversal potential measurements of single-channel currents through 10 pS and 20 pS anion channels in human airway epithelial cells. The sequences obtained were Cl- > I- > Br- ≧ F- for the 20 pS channel and Cl- > I- ≧ Br- ≧ F- for the 20 pS channel. However, the permeability differences were not large, the greatest being 0.66 for the ratio of fluoride to chloride permeability in the 20 pS channel. Single-channel currents were also measured with solutions of constant haiide concentration but varying ratios of chloride to fluoride ions. An anomalous mole fraction effect was observed for the 20 pS channel but not for the 10 pS channel, suggesting that the former is a multi-ion channel. Comparison of the halide permeability sequences of these two channels with those of whole-cell currents in other epithelial cells does not support their involvement in any of the known whole-cell epithelial currents.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 160-166 |
| Nombre de pages | 7 |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1104 |
| Numéro de publication | 1 |
| DOI | |
| Statut de publication | Published - févr. 17 1992 |
| Publié à l'externe | Oui |
Note bibliographique
Funding Information:We thank Marek Duszyk for advice and encouragement throughout this work, Paul Labrecque for assistance in cell preparation, and the Department of Otolaryngology of the University of Alberta Hospital for providing human tissues. This work was supported by the Medical Research Council of Canada and the Canadian Cystic Fibrosis Foundation.
ASJC Scopus Subject Areas
- Biophysics
- Biochemistry
- Cell Biology