Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer of glucose uptake and modulator of insulin signaling-associated pathways

Yu Ting Kuo, Chih Chan Lin, Hsiao Tzu Kuo, Jui Hsiang Hung, Ching Hsuan Liu, Alagie Jassey, Ming Hong Yen, Shu Jing Wu, Liang Tzung Lin

Résultat de recherche: Articleexamen par les pairs

32 Citations (Scopus)

Résumé

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies were evaluated in differentiated C2C12 skeletal muscle cells, and HPLC analysis was used to determine the molecular bioactive constituents. Our results indicated that the ethanolic extracts of BOF and DAI (BOFEE and DAIEE, respectively) enhanced the glucose uptake ratio in the differentiated C2C12 cells, and further analysis identified the flavone baicalin as a major constituent capable of efficiently stimulating glucose absorption. Mechanistic studies revealed that the effect from baicalin involved the activation of IRS-1 and GLUT-4, and implicated the AMPK, PI3K/Akt, and MAPK/ERK signaling cascades. Due to its potency, we suggest that baicalin merit further evaluation as a potential candidate anti-hyperglycemic agent for the treatment and management of T2DM.

Langue d'origineEnglish
Pages (de-à)240-248
Nombre de pages9
JournalJournal of Food and Drug Analysis
Volume27
Numéro de publication1
DOI
Statut de publicationPublished - janv. 2019

Note bibliographique

Funding Information:
YTK and LTL were supported by a grant from Chi Mei Medical Center and Taipei Medical University (105CM-TMU-06); SJW was funded by the Department of Chinese Medicine and Pharmacy, Ministry of Health and Welfare of Taiwan (CCMP101-RD-018).

Funding Information:
YTK and LTL were supported by a grant from Chi Mei Medical Center and Taipei Medical University ( 105CM-TMU-06 ); SJW was funded by the Department of Chinese Medicine and Pharmacy, Ministry of Health and Welfare of Taiwan ( CCMP101-RD-018 ).

Publisher Copyright:
© 2018

ASJC Scopus Subject Areas

  • Food Science
  • Pharmacology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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