In situ identification and N2 and C fixation rates of uncultivated cyanobacteria populations

Andreas Krupke; Niculina Musat; Julie LaRoche; Wiebke Mohr; Bernhard M. Fuchs; Rudolf I. Amann; Marcel M.M. Kuypers; Rachel A. Foster

doi:10.1016/j.syapm.2013.02.002

In situ identification and N₂ and C fixation rates of uncultivated cyanobacteria populations

Andreas Krupke, Niculina Musat, Julie LaRoche, Wiebke Mohr, Bernhard M. Fuchs, Rudolf I. Amann, Marcel M.M. Kuypers, Rachel A. Foster

Résultat de recherche: Article › examen par les pairs

61 Citations (Scopus)

Résumé

Nitrogen (N₂) fixation is a globally important process often mediated by diazotrophic cyanobacteria in the open ocean. In 2010, seawater was collected near Cape Verde to identify and measure N₂ and carbon (C) fixation by unicellular diazotrophic cyanobacteria. The nifH gene abundance (10⁴-10⁶nifHL^-1) and nifH gene transcript abundance (10²-10⁴cDNAnifHL^-1) for two unicellular groups, UCYN-A and UCYN-B, were detected. UCYN-A was also identified and quantified (10⁴-10⁵cellsL^-1) by new probes (UCYN-A732 and UCYN-A159) using Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARD-FISH) assays. The UCYN-A were observed as free cells or attached to a larger unidentified eukaryotic cell. A Halogen In Situ Hybridization-Secondary Ion Mass Spectrometry (HISH-SIMS) assay using the UCYN-A732 probe was applied on samples previously incubated with ¹³C-bicarbonate and ¹⁵N₂. Free UCYN-A cells were enriched in both ¹³C and ¹⁵N and estimated C and N₂ fixation rates for UCYN-A were lower compared to co-occurring unicellular cyanobacteria cells similar in size (3.1-5.6μm) and pigmentation to diazotroph Crocosphaera watsonii. Here, we identify and quantify two common co-occurring unicellular groups and measure their cellular activities by nanoSIMS.

Langue d'origine	English
Pages (de-à)	259-271
Nombre de pages	13
Journal	Systematic and Applied Microbiology
Volume	36
Numéro de publication	4
DOI	https://doi.org/10.1016/j.syapm.2013.02.002
Statut de publication	Published - juin 2013

Note bibliographique

Funding Information:
The authors would like to thank Jörg Wulf, Daniela Franzke, Abdul Rahiman Sheik, Birgit Adam, Gaute Lavik, Thomas Max and Gabriele Klockgether for their technical assistance. We thank Jonathan P. Zehr, Brandon Carter, and Anne Thompson of the University of California for samples used to test our assays and many discussions on results presented herein. We thank two anonymous reviewers for their comments and helpful suggestions for improving our manuscript. We would also like to thank Hannah Halm and Philip Raab for their assistance and the captain and crew of the Synagoga and R/V Islandia for their help during sample collections in 2008 and 2010. A special acknowledgement to Oscar Melicio, Carlos Ferreira Santos, Pericles Silva, Ivanice Monteiro, and Nuno Vieira of the Insituto Nacional de Deesenvolvimento das Pescas (INDP), Cape Verde, for hosting and assisting our expedition. We thank the captain and crew of the R/V Maria S. Merian for help with sampling during the MSM 03/1 VISION from Island to the Azores expedition. AK also thanks Sara J. Bender for her helpful comments and discussions of the manuscript. This work was supported by the Max Planck Society and JL and WM thank the Bundesministerium für Bildung und Forschung (BMBF) for financial support through the SOPRAN II (Surface Ocean Processes in the Anthropocene) project, grant number.

ASJC Scopus Subject Areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Applied Microbiology and Biotechnology

Accès au document

10.1016/j.syapm.2013.02.002

Autres fichiers et liens

Citer

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title = "In situ identification and N2 and C fixation rates of uncultivated cyanobacteria populations",

abstract = "Nitrogen (N2) fixation is a globally important process often mediated by diazotrophic cyanobacteria in the open ocean. In 2010, seawater was collected near Cape Verde to identify and measure N2 and carbon (C) fixation by unicellular diazotrophic cyanobacteria. The nifH gene abundance (104-106nifHL-1) and nifH gene transcript abundance (102-104cDNAnifHL-1) for two unicellular groups, UCYN-A and UCYN-B, were detected. UCYN-A was also identified and quantified (104-105cellsL-1) by new probes (UCYN-A732 and UCYN-A159) using Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARD-FISH) assays. The UCYN-A were observed as free cells or attached to a larger unidentified eukaryotic cell. A Halogen In Situ Hybridization-Secondary Ion Mass Spectrometry (HISH-SIMS) assay using the UCYN-A732 probe was applied on samples previously incubated with 13C-bicarbonate and 15N2. Free UCYN-A cells were enriched in both 13C and 15N and estimated C and N2 fixation rates for UCYN-A were lower compared to co-occurring unicellular cyanobacteria cells similar in size (3.1-5.6μm) and pigmentation to diazotroph Crocosphaera watsonii. Here, we identify and quantify two common co-occurring unicellular groups and measure their cellular activities by nanoSIMS.",

author = "Andreas Krupke and Niculina Musat and Julie LaRoche and Wiebke Mohr and Fuchs, {Bernhard M.} and Amann, {Rudolf I.} and Kuypers, {Marcel M.M.} and Foster, {Rachel A.}",

note = "Funding Information: The authors would like to thank J{\"o}rg Wulf, Daniela Franzke, Abdul Rahiman Sheik, Birgit Adam, Gaute Lavik, Thomas Max and Gabriele Klockgether for their technical assistance. We thank Jonathan P. Zehr, Brandon Carter, and Anne Thompson of the University of California for samples used to test our assays and many discussions on results presented herein. We thank two anonymous reviewers for their comments and helpful suggestions for improving our manuscript. We would also like to thank Hannah Halm and Philip Raab for their assistance and the captain and crew of the Synagoga and R/V Islandia for their help during sample collections in 2008 and 2010. A special acknowledgement to Oscar Melicio, Carlos Ferreira Santos, Pericles Silva, Ivanice Monteiro, and Nuno Vieira of the Insituto Nacional de Deesenvolvimento das Pescas (INDP), Cape Verde, for hosting and assisting our expedition. We thank the captain and crew of the R/V Maria S. Merian for help with sampling during the MSM 03/1 VISION from Island to the Azores expedition. AK also thanks Sara J. Bender for her helpful comments and discussions of the manuscript. This work was supported by the Max Planck Society and JL and WM thank the Bundesministerium f{\"u}r Bildung und Forschung (BMBF) for financial support through the SOPRAN II (Surface Ocean Processes in the Anthropocene) project, grant number. ",

year = "2013",

month = jun,

doi = "10.1016/j.syapm.2013.02.002",

language = "English",

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pages = "259--271",

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T1 - In situ identification and N2 and C fixation rates of uncultivated cyanobacteria populations

AU - Krupke, Andreas

AU - Musat, Niculina

AU - LaRoche, Julie

AU - Mohr, Wiebke

AU - Fuchs, Bernhard M.

AU - Amann, Rudolf I.

AU - Kuypers, Marcel M.M.

AU - Foster, Rachel A.

N1 - Funding Information: The authors would like to thank Jörg Wulf, Daniela Franzke, Abdul Rahiman Sheik, Birgit Adam, Gaute Lavik, Thomas Max and Gabriele Klockgether for their technical assistance. We thank Jonathan P. Zehr, Brandon Carter, and Anne Thompson of the University of California for samples used to test our assays and many discussions on results presented herein. We thank two anonymous reviewers for their comments and helpful suggestions for improving our manuscript. We would also like to thank Hannah Halm and Philip Raab for their assistance and the captain and crew of the Synagoga and R/V Islandia for their help during sample collections in 2008 and 2010. A special acknowledgement to Oscar Melicio, Carlos Ferreira Santos, Pericles Silva, Ivanice Monteiro, and Nuno Vieira of the Insituto Nacional de Deesenvolvimento das Pescas (INDP), Cape Verde, for hosting and assisting our expedition. We thank the captain and crew of the R/V Maria S. Merian for help with sampling during the MSM 03/1 VISION from Island to the Azores expedition. AK also thanks Sara J. Bender for her helpful comments and discussions of the manuscript. This work was supported by the Max Planck Society and JL and WM thank the Bundesministerium für Bildung und Forschung (BMBF) for financial support through the SOPRAN II (Surface Ocean Processes in the Anthropocene) project, grant number.

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N2 - Nitrogen (N2) fixation is a globally important process often mediated by diazotrophic cyanobacteria in the open ocean. In 2010, seawater was collected near Cape Verde to identify and measure N2 and carbon (C) fixation by unicellular diazotrophic cyanobacteria. The nifH gene abundance (104-106nifHL-1) and nifH gene transcript abundance (102-104cDNAnifHL-1) for two unicellular groups, UCYN-A and UCYN-B, were detected. UCYN-A was also identified and quantified (104-105cellsL-1) by new probes (UCYN-A732 and UCYN-A159) using Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARD-FISH) assays. The UCYN-A were observed as free cells or attached to a larger unidentified eukaryotic cell. A Halogen In Situ Hybridization-Secondary Ion Mass Spectrometry (HISH-SIMS) assay using the UCYN-A732 probe was applied on samples previously incubated with 13C-bicarbonate and 15N2. Free UCYN-A cells were enriched in both 13C and 15N and estimated C and N2 fixation rates for UCYN-A were lower compared to co-occurring unicellular cyanobacteria cells similar in size (3.1-5.6μm) and pigmentation to diazotroph Crocosphaera watsonii. Here, we identify and quantify two common co-occurring unicellular groups and measure their cellular activities by nanoSIMS.

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