Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin

Takashi Hirama, Gregory D. Fairn

Résultat de recherche: Articleexamen par les pairs

14 Citations (Scopus)

Résumé

Membrane curvature is a property of biological membranes essential for organelle morphology and the formation of tubulovesicular carriers. Curvature generation is influenced by the lipid composition of the membrane and protein-mediated processes. Lipids with small headgroups, such as phosphatidic acid, are conical and impose negative curvature on a monolayer. Conversely, lipids with large headgroups relative to the hydrophobic tail(s), such as lysophosphatidylcholine, have an inverted conical shape and impose positive curvature. Due to its abundance and high rates of spontaneous flip-flop between membrane leaflets cholesterol is proposed to buffer the formation of membrane curvature. Recently, we demonstrated that cholesterol is also crucial for maintaining the proper spacing of anionic phospholipids. Upon extraction of cholesterol with cyclodextrin there is a sharp increase in the negative surface charge density of the plasma membrane, which promotes electrostatic repulsion between anionic headgroups, the generation of spontaneous positive curvature and rapid membrane internalization.

Langue d'origineEnglish
Pages (de-à)1-4
Nombre de pages4
JournalCommunicative and Integrative Biology
Volume11
Numéro de publication2
DOI
Statut de publicationPublished - mars 4 2018
Publié à l'externeOui

Note bibliographique

Funding Information:
for this research was provided by a New Investigator Award and an Operating Grant from the Canadian Institutes of Health Research to G.D.F (grant no. MOP-133656).

Funding Information:
Funding for this research was provided by a New Investigator Award and an Operating Grant from the Canadian Institutes of Health Research to G.D.F (grant no. MOP-133656).

Publisher Copyright:
© 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

ASJC Scopus Subject Areas

  • General Agricultural and Biological Sciences

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