TY - JOUR
T1 - Induction of yeast histone genes by stimulation of stationary-phase cells
AU - Drebot, Michael A.
AU - Veinot-Drebot, Lela M.
AU - Singer, Richard A.
AU - Johnston, Gerald C.
PY - 1990
Y1 - 1990
N2 - In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that is unrelated to the mitotic cell cycle. Expression of histone genes, as assessed by Northern (RNA) analysis, was shown to increase promptly after the stimulation, brought about by fresh medium, that activates stationary-phase cells to reenter the mitotic cell cycle. The use of a yeast mutant that is conditionally blocked in the resumption of proliferation at a step that is not part of the mitotic cell cycle (M. A. Drebot, G. C. Johnston, and R. A. Singer, Proc. Natl. Acad. Sci. 84:7948, 1987) showed that this increased gene expression that occurs upon stimulation of stationary-phase cells took place in the absence of DNA synthesis and without the performance of start. This stimulation-specific gene expression was blocked by the mating pheromone α-factor, indicating that α-factor directly inhibits expression of these histone genes, independently of start.
AB - In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that is unrelated to the mitotic cell cycle. Expression of histone genes, as assessed by Northern (RNA) analysis, was shown to increase promptly after the stimulation, brought about by fresh medium, that activates stationary-phase cells to reenter the mitotic cell cycle. The use of a yeast mutant that is conditionally blocked in the resumption of proliferation at a step that is not part of the mitotic cell cycle (M. A. Drebot, G. C. Johnston, and R. A. Singer, Proc. Natl. Acad. Sci. 84:7948, 1987) showed that this increased gene expression that occurs upon stimulation of stationary-phase cells took place in the absence of DNA synthesis and without the performance of start. This stimulation-specific gene expression was blocked by the mating pheromone α-factor, indicating that α-factor directly inhibits expression of these histone genes, independently of start.
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U2 - 10.1128/MCB.10.12.6356
DO - 10.1128/MCB.10.12.6356
M3 - Article
C2 - 2247060
AN - SCOPUS:0025201312
SN - 0270-7306
VL - 10
SP - 6356
EP - 6361
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 12
ER -