Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection

Hai Ying Zhang, Amin A. Nanji, John M. Luk, Xiao Ru Huang, Chung Mau Lo, Yong Xiong Chen, Siu Tsan Yuen, Hui Y. Lan, George K.K. Lau

Résultat de recherche: Articleexamen par les pairs

22 Citations (Scopus)

Résumé

Background: Macrophage migration inhibitory factor (MIF) has emerged to be a pivotal cytokine in immune-mediated diseases. Patients and methods: To investigate the role of MIF in chronic hepatitis B infection, we studied two groups of hepatitis B surface antigen positive patients: group 1 (immune tolerant, n = 16) and group 2 (immune clearance, n = 16). Serum level of MIF was measured by enzyme-linked immunosorbent assay and intrahepatic expression of MIF, macrophage and T-cell localisation were detected by double immunohistochemistry. Results: An increased serum MIF correlated significantly with increased serum alanine aminotransferase activity (r = 0.73, P < 0.001) and the severity of necroinflammatory injury (r = 0.642, P < 0.001). In group 2, there was marked MIF mRNA expression in all KP-1+ macrophages and CD45RO+ activated T cells and, to a lesser extent, in hepatocytes within inflammatory areas. In contrast to its mRNA expression, the cytoplasmic MIF protein level in hepatocytes, infiltrating macrophages and T cells within the inflammatory area was reduced, which probably contributed to the increased serum MIF level. Conclusions: Our data suggested that MIF played a role in sustaining cell-mediated hepatic injury during the immune-clearance phase of chronic hepatitis B infection.

Langue d'origineEnglish
Pages (de-à)571-579
Nombre de pages9
JournalLiver International
Volume25
Numéro de publication3
DOI
Statut de publicationPublished - juin 2005
Publié à l'externeOui

ASJC Scopus Subject Areas

  • Hepatology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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