Mapping quantitative trait loci for open-field behavior in mice

Howard K. Gershenfeld; Paul E. Neumann; Chantal Mathis; Jacqueline N. Crawley; Xiaohua Li; Steven M. Paul

doi:10.1023/A:1025653812535

Mapping quantitative trait loci for open-field behavior in mice

Howard K. Gershenfeld, Paul E. Neumann, Chantal Mathis, Jacqueline N. Crawley, Xiaohua Li, Steven M. Paul

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

154 Citations (Scopus)

Résumé

By performing a whole genome screen in an F₂ intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F₂ population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.

Langue d'origine	English
Pages (de-à)	201-210
Nombre de pages	10
Journal	Behavior Genetics
Volume	27
Numéro de publication	3
DOI	https://doi.org/10.1023/A:1025653812535
Statut de publication	Published - 1997

Note bibliographique

Funding Information:
H.G. would like to thank C. B. Taylor, I. L. Weissman, and D. Baltimore for encouragement in the larval stages of this project. We appreciated helpful conversations with E. Remmers, J. Thomas, E. Ginns, R. Philibert, W. Dietrich, E. Lander, C. Basten, and Z. B. Zeng, our NIH and UTSW colleagues. We are grateful to the NIMH Computer support group, the Whitehead/MIT Mouse Genome Project, Research Genetics, and the Mouse Genome Informatics Project providing infrastructure for this work. This project has been generously supported by NIMH-LRP, a NARSAD Young Investigators Award (H.G.), the Southwestern Medical Foundation (H.G.), the KZA Hope Fund (H.G.), and the Medical Research Council of Canada (P.E.N.).

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics
Genetics(clinical)

Accès au document

10.1023/A:1025653812535

Autres fichiers et liens

Citer

@article{5d18ddc3cd7c4f90967b2dbce0da042f,

title = "Mapping quantitative trait loci for open-field behavior in mice",

abstract = "By performing a whole genome screen in an F2 intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F2 population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.",

author = "Gershenfeld, {Howard K.} and Neumann, {Paul E.} and Chantal Mathis and Crawley, {Jacqueline N.} and Xiaohua Li and Paul, {Steven M.}",

note = "Funding Information: H.G. would like to thank C. B. Taylor, I. L. Weissman, and D. Baltimore for encouragement in the larval stages of this project. We appreciated helpful conversations with E. Remmers, J. Thomas, E. Ginns, R. Philibert, W. Dietrich, E. Lander, C. Basten, and Z. B. Zeng, our NIH and UTSW colleagues. We are grateful to the NIMH Computer support group, the Whitehead/MIT Mouse Genome Project, Research Genetics, and the Mouse Genome Informatics Project providing infrastructure for this work. This project has been generously supported by NIMH-LRP, a NARSAD Young Investigators Award (H.G.), the Southwestern Medical Foundation (H.G.), the KZA Hope Fund (H.G.), and the Medical Research Council of Canada (P.E.N.).",

year = "1997",

doi = "10.1023/A:1025653812535",

language = "English",

volume = "27",

pages = "201--210",

journal = "Behavior Genetics",

issn = "0001-8244",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - Mapping quantitative trait loci for open-field behavior in mice

AU - Gershenfeld, Howard K.

AU - Neumann, Paul E.

AU - Mathis, Chantal

AU - Crawley, Jacqueline N.

AU - Li, Xiaohua

AU - Paul, Steven M.

N1 - Funding Information: H.G. would like to thank C. B. Taylor, I. L. Weissman, and D. Baltimore for encouragement in the larval stages of this project. We appreciated helpful conversations with E. Remmers, J. Thomas, E. Ginns, R. Philibert, W. Dietrich, E. Lander, C. Basten, and Z. B. Zeng, our NIH and UTSW colleagues. We are grateful to the NIMH Computer support group, the Whitehead/MIT Mouse Genome Project, Research Genetics, and the Mouse Genome Informatics Project providing infrastructure for this work. This project has been generously supported by NIMH-LRP, a NARSAD Young Investigators Award (H.G.), the Southwestern Medical Foundation (H.G.), the KZA Hope Fund (H.G.), and the Medical Research Council of Canada (P.E.N.).

PY - 1997

Y1 - 1997

N2 - By performing a whole genome screen in an F2 intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F2 population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.

AB - By performing a whole genome screen in an F2 intercross of two strains of mice (A/J and C57BL/6J), which differ markedly in their behavioral response to a brightly lit open field (O-F) we have mapped several quantitative trait loci (QTL) for this complex behavioral phenotype. QTL on chromosomes 1 and 10 were identified that affect both initial ambulation in the O-F (initial 'response to novelty' ambulation) (lod of 7.1 and 8.8, respectively) and vertical rearings (lod of 4.5 and 8.5, respectively). For habituated O-F behavior, QTL were identified on chromosomes 3 and 10 for ambulation (lod of 4.1 and 14.7, respectively) and on chromosomes 1, 10, and 19 for vertical rearings (lod of 5.8, 6.0, and 4.7, respectively). The QTL on chromosome 1 (near D1Mit116; 101 cM) was specific for initial O-F ambulation behavior, whereas the QTL on chromosome 10 (near D10Mit237; 74 cM) affected both initial and habituated rearing behavior. Additional suggestive QTL (lod, >2.8) were mapped to chromosomes 1, 8, 11, 15, and 19. The QTL on chromosomes 1, 10, and 19 individually explain from 3.2 to 12.7%. Collectively, the multiple independent QTL explain from 16.3 to 24.1% of the F2 population's phenotypic variance, depending on the trait. These identified QTL should prove useful for dissecting the genetic and behavioral dimensions of O-F behavior, fostering an understanding of individual differences.

UR - http://www.scopus.com/inward/record.url?scp=0030999378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030999378&partnerID=8YFLogxK

U2 - 10.1023/A:1025653812535

DO - 10.1023/A:1025653812535

M3 - Article

C2 - 9210791

AN - SCOPUS:0030999378

SN - 0001-8244

VL - 27

SP - 201

EP - 210

JO - Behavior Genetics

JF - Behavior Genetics

IS - 3

ER -

Mapping quantitative trait loci for open-field behavior in mice

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

Citer