Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro

E. A. MacKinnon; J. F. Henderson; D. B. Stoltz; P. Faulkner

doi:10.1016/S0022-5320(74)90055-0

Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro

E. A. MacKinnon, J. F. Henderson, D. B. Stoltz, P. Faulkner

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80 Citations (Scopus)

Résumé

An ultrastructural description is given of nuclear polyhedrosis virus (NPV) morphogenesis in vitro, with emphasis on changes occurring during long-term serial passage of infectious material. The morphogenesis and infectivity of early passage virus is similar to that which obtains in vivo. The infectivity of polyhedra prepared from late passage infected cultures is greatly diminished; this is paralleled by both a decrease in the total number of polyhedra produced and a decrease in the number of morphologically normal occluded virus particles. Late passage viral morphogenesis is profoundly changed. New observations concerning the acquisition of membranes by NPV nucleocapsids and polyhedron formation are discussed.

Langue d'origine	English
Pages (de-à)	419-435
Nombre de pages	17
Journal	Journal of Structural Biology
Volume	49
Numéro de publication	3
DOI	https://doi.org/10.1016/S0022-5320(74)90055-0
Statut de publication	Published - déc. 1974
Publié à l'externe	Oui

Note bibliographique

Funding Information:
We thank Dr. R. P. Jaques, Canada Agriculture Station, Harrow, Ontario for providing facilities for in vivo infectivity tests. This work was supported by a grant from the National Research Council, Canada.

ASJC Scopus Subject Areas

Anatomy
Molecular Biology

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10.1016/S0022-5320(74)90055-0

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abstract = "An ultrastructural description is given of nuclear polyhedrosis virus (NPV) morphogenesis in vitro, with emphasis on changes occurring during long-term serial passage of infectious material. The morphogenesis and infectivity of early passage virus is similar to that which obtains in vivo. The infectivity of polyhedra prepared from late passage infected cultures is greatly diminished; this is paralleled by both a decrease in the total number of polyhedra produced and a decrease in the number of morphologically normal occluded virus particles. Late passage viral morphogenesis is profoundly changed. New observations concerning the acquisition of membranes by NPV nucleocapsids and polyhedron formation are discussed.",

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AU - MacKinnon, E. A.

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AU - Stoltz, D. B.

AU - Faulkner, P.

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AB - An ultrastructural description is given of nuclear polyhedrosis virus (NPV) morphogenesis in vitro, with emphasis on changes occurring during long-term serial passage of infectious material. The morphogenesis and infectivity of early passage virus is similar to that which obtains in vivo. The infectivity of polyhedra prepared from late passage infected cultures is greatly diminished; this is paralleled by both a decrease in the total number of polyhedra produced and a decrease in the number of morphologically normal occluded virus particles. Late passage viral morphogenesis is profoundly changed. New observations concerning the acquisition of membranes by NPV nucleocapsids and polyhedron formation are discussed.

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Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro

Résumé

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ASJC Scopus Subject Areas

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