TY - GEN
T1 - Novel rheology of the human airway smooth muscle cell
AU - Maksym, G. N.
AU - Fabry, B.
AU - Navajas, D.
AU - Butler, J.
AU - Fredberg, J. J.
PY - 1999
Y1 - 1999
N2 - We have developed a method for determining the mechanical impedance of the cytoskeleton (CSK) based on magnetic twisting cytometry. Ligand coated ferromagnetic beads were bound to the integrin receptors of human airway smooth muscle cells, and oscillatory torques applied with a magnetic field. Bead rotation was determined by measuring the oscillating magnetic field produced by the beads. The time course of stiffening in response to pharmacological agonists was also measured with a resolution of 10s. Apparent elastance (E) and viscosity (R) of the CSK were calculated from the complex ratio of specific magnetic torque to bead rotation. E increased weakly with oscillation frequency while R decreased inversely with frequency. Although the magnitudes of E and R were altered during changes in muscle activation, the frequency dependence was unchanged. This frequency dependence is known as structural damping, and is a novel finding for the mechanics of the CSK.
AB - We have developed a method for determining the mechanical impedance of the cytoskeleton (CSK) based on magnetic twisting cytometry. Ligand coated ferromagnetic beads were bound to the integrin receptors of human airway smooth muscle cells, and oscillatory torques applied with a magnetic field. Bead rotation was determined by measuring the oscillating magnetic field produced by the beads. The time course of stiffening in response to pharmacological agonists was also measured with a resolution of 10s. Apparent elastance (E) and viscosity (R) of the CSK were calculated from the complex ratio of specific magnetic torque to bead rotation. E increased weakly with oscillation frequency while R decreased inversely with frequency. Although the magnitudes of E and R were altered during changes in muscle activation, the frequency dependence was unchanged. This frequency dependence is known as structural damping, and is a novel finding for the mechanics of the CSK.
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M3 - Conference contribution
AN - SCOPUS:0033357885
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 64
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -