Résumé
In Crithidia fasciculata, a trypanosomatid protozoan, the large ribosomal subunit contains five small RNA species (e, f, g, i, j) in addition to 5S rRNA [Gray, M.W. (1981) Mol. Cell, Biol. 1, 347-357]. The complete primary sequence of species i is shown here to be pAACGUGUmCGCGAUGGAUGACUUGGCUUCCUAUCUCGUUGA... AGAmACGCAGUSAAGUGCGAUAAGUGGUAψCAAUUGmCAGAAUCAUUCAAUUACCGAAUCUUUGAACGCAAACGG... CGCAUGGGAGAAGCUCUUUUGAGUCAUCCCCGUGCAUGCCAUAUUCUCCAmGUGUCGAA(C)OH. This sequence establishes that species i is a 5.8S rRNA, despite its exceptional length (171-172 nucleotides). The extra nucleotides in C. fasciculata 5.8S rRNA are located in a region whose primary sequence and length are highly variable among 5.8S rRNAs, but which is capable of forming a stable hairpin loop structure (the "G+C-rich hairpin"). The sequence of C. fasciculata 5.8S rRNA is no more closely related to that of another protozoan, Acanthamoeba castellanii , than it is to representative 5,8S rRNA sequences from the other eukaryotic kingdoms, emphasizing the deep phylogenetic divisions that seem to exist within the Kingdom Protista.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 2085-2092 |
| Nombre de pages | 8 |
| Journal | Nucleic Acids Research |
| Volume | 10 |
| Numéro de publication | 6 |
| DOI | |
| Statut de publication | Published - mars 25 1982 |
Note bibliographique
Funding Information:data prior to publication. We are grateful to R.M. MacKay for a gift of unlabelled C. faacicv.la.ta 5.8S rRNA. This work was supported by a grant from the Medical Research Council of Canada to M.W.G.
ASJC Scopus Subject Areas
- Genetics