Piperine inhibits the growth and motility of triple-negative breast cancer cells

Anna L. Greenshields; Carolyn D. Doucette; Kimberly M. Sutton; Laurence Madera; Henry Annan; Paul B. Yaffe; Allison F. Knickle; Zhongmin Dong; David W. Hoskin

doi:10.1016/j.canlet.2014.11.017

Piperine inhibits the growth and motility of triple-negative breast cancer cells

Anna L. Greenshields, Carolyn D. Doucette, Kimberly M. Sutton, Laurence Madera, Henry Annan, Paul B. Yaffe, Allison F. Knickle, Zhongmin Dong, David W. Hoskin

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Résumé

Piperine, an alkaloid from black pepper, is reported to have anticancer activities. In this study, we investigated the effect of piperine on the growth and motility of triple-negative breast cancer (TNBC) cells. Piperine inhibited the in vitro growth of TNBC cells, as well as hormone-dependent breast cancer cells, without affecting normal mammary epithelial cell growth. Exposure to piperine decreased the percentage of TNBC cells in the G2 phase of the cell cycle. In addition, G1- and G2-associated protein expression was decreased and p21^Waf1/Cip1 expression was increased in piperine-treated TNBC cells. Piperine also inhibited survival-promoting Akt activation in TNBC cells and caused caspase-dependent apoptosis via the mitochondrial pathway. Interestingly, combined treatment with piperine and γ radiation was more cytotoxic for TNBC cells than γ radiation alone. The in vitro migration of piperine-treated TNBC cells was impaired and expression of matrix metalloproteinase-2 and -9 mRNA was decreased, suggesting an antimetastatic effect by piperine. Finally, intratumoral administration of piperine inhibited the growth of TNBC xenografts in immune-deficient mice. Taken together, these findings suggest that piperine may be useful in the treatment of TNBC.

Langue d'origine	English
Pages (de-à)	129-140
Nombre de pages	12
Journal	Cancer Letters
Volume	357
Numéro de publication	1
DOI	https://doi.org/10.1016/j.canlet.2014.11.017
Statut de publication	Published - févr. 1 2015

Note bibliographique

Funding Information:
This work was supported by a grant ( R12F13 ) to D. Hoskin from the Canadian Breast Cancer Foundation-Atlantic Region . C. Doucette, A. Greenshields, and K. Sutton were recipients of Natural Sciences and Engineering Research Council Postgraduate Studentships. C. Doucette was also supported by a Nova Scotia Health Research Foundation Studentship. A. Greenshields, K. Sutton, L. Madera, and A. Knickle were recipients of Trainee Awards from the Cancer Research Training Program . L. Madera was also supported by a Canadian Breast Cancer Foundation-Atlantic Region Postdoctoral Fellowship. P. Yaffe was the recipient of a Resident Research Award from the Department of Surgery , a Killam Scholarship , and a Nova Scotia Health Research Foundation Studentship. H. Annan was supported by a Natural Sciences and Engineering Research Council Undergraduate Student Research Award.

Publisher Copyright:
© 2014 Elsevier Ireland Ltd.

ASJC Scopus Subject Areas

Oncology
Cancer Research

ODD de l’ONU

Cette action contribue à la réalisation des objectifs de développement durable suivants

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10.1016/j.canlet.2014.11.017

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title = "Piperine inhibits the growth and motility of triple-negative breast cancer cells",

abstract = "Piperine, an alkaloid from black pepper, is reported to have anticancer activities. In this study, we investigated the effect of piperine on the growth and motility of triple-negative breast cancer (TNBC) cells. Piperine inhibited the in vitro growth of TNBC cells, as well as hormone-dependent breast cancer cells, without affecting normal mammary epithelial cell growth. Exposure to piperine decreased the percentage of TNBC cells in the G2 phase of the cell cycle. In addition, G1- and G2-associated protein expression was decreased and p21Waf1/Cip1 expression was increased in piperine-treated TNBC cells. Piperine also inhibited survival-promoting Akt activation in TNBC cells and caused caspase-dependent apoptosis via the mitochondrial pathway. Interestingly, combined treatment with piperine and γ radiation was more cytotoxic for TNBC cells than γ radiation alone. The in vitro migration of piperine-treated TNBC cells was impaired and expression of matrix metalloproteinase-2 and -9 mRNA was decreased, suggesting an antimetastatic effect by piperine. Finally, intratumoral administration of piperine inhibited the growth of TNBC xenografts in immune-deficient mice. Taken together, these findings suggest that piperine may be useful in the treatment of TNBC.",

author = "Greenshields, {Anna L.} and Doucette, {Carolyn D.} and Sutton, {Kimberly M.} and Laurence Madera and Henry Annan and Yaffe, {Paul B.} and Knickle, {Allison F.} and Zhongmin Dong and Hoskin, {David W.}",

note = "Funding Information: This work was supported by a grant ( R12F13 ) to D. Hoskin from the Canadian Breast Cancer Foundation-Atlantic Region . C. Doucette, A. Greenshields, and K. Sutton were recipients of Natural Sciences and Engineering Research Council Postgraduate Studentships. C. Doucette was also supported by a Nova Scotia Health Research Foundation Studentship. A. Greenshields, K. Sutton, L. Madera, and A. Knickle were recipients of Trainee Awards from the Cancer Research Training Program . L. Madera was also supported by a Canadian Breast Cancer Foundation-Atlantic Region Postdoctoral Fellowship. P. Yaffe was the recipient of a Resident Research Award from the Department of Surgery , a Killam Scholarship , and a Nova Scotia Health Research Foundation Studentship. H. Annan was supported by a Natural Sciences and Engineering Research Council Undergraduate Student Research Award. Publisher Copyright: {\textcopyright} 2014 Elsevier Ireland Ltd.",

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AU - Yaffe, Paul B.

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AU - Dong, Zhongmin

AU - Hoskin, David W.

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N2 - Piperine, an alkaloid from black pepper, is reported to have anticancer activities. In this study, we investigated the effect of piperine on the growth and motility of triple-negative breast cancer (TNBC) cells. Piperine inhibited the in vitro growth of TNBC cells, as well as hormone-dependent breast cancer cells, without affecting normal mammary epithelial cell growth. Exposure to piperine decreased the percentage of TNBC cells in the G2 phase of the cell cycle. In addition, G1- and G2-associated protein expression was decreased and p21Waf1/Cip1 expression was increased in piperine-treated TNBC cells. Piperine also inhibited survival-promoting Akt activation in TNBC cells and caused caspase-dependent apoptosis via the mitochondrial pathway. Interestingly, combined treatment with piperine and γ radiation was more cytotoxic for TNBC cells than γ radiation alone. The in vitro migration of piperine-treated TNBC cells was impaired and expression of matrix metalloproteinase-2 and -9 mRNA was decreased, suggesting an antimetastatic effect by piperine. Finally, intratumoral administration of piperine inhibited the growth of TNBC xenografts in immune-deficient mice. Taken together, these findings suggest that piperine may be useful in the treatment of TNBC.

AB - Piperine, an alkaloid from black pepper, is reported to have anticancer activities. In this study, we investigated the effect of piperine on the growth and motility of triple-negative breast cancer (TNBC) cells. Piperine inhibited the in vitro growth of TNBC cells, as well as hormone-dependent breast cancer cells, without affecting normal mammary epithelial cell growth. Exposure to piperine decreased the percentage of TNBC cells in the G2 phase of the cell cycle. In addition, G1- and G2-associated protein expression was decreased and p21Waf1/Cip1 expression was increased in piperine-treated TNBC cells. Piperine also inhibited survival-promoting Akt activation in TNBC cells and caused caspase-dependent apoptosis via the mitochondrial pathway. Interestingly, combined treatment with piperine and γ radiation was more cytotoxic for TNBC cells than γ radiation alone. The in vitro migration of piperine-treated TNBC cells was impaired and expression of matrix metalloproteinase-2 and -9 mRNA was decreased, suggesting an antimetastatic effect by piperine. Finally, intratumoral administration of piperine inhibited the growth of TNBC xenografts in immune-deficient mice. Taken together, these findings suggest that piperine may be useful in the treatment of TNBC.

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Piperine inhibits the growth and motility of triple-negative breast cancer cells

Résumé

Note bibliographique

ASJC Scopus Subject Areas

ODD de l’ONU

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