Planomonadida ord. nov. (Apusozoa): Ultrastructural Affinity with Micronuclearia podoventralis and Deep Divergences within Planomonas gen. nov.

Thomas Cavalier-Smith; Ema E. Chao; Alexandra Stechmann; Brian Oates; Sergei Nikolaev

doi:10.1016/j.protis.2008.06.002

Planomonadida ord. nov. (Apusozoa): Ultrastructural Affinity with Micronuclearia podoventralis and Deep Divergences within Planomonas gen. nov.

Thomas Cavalier-Smith, Ema E. Chao, Alexandra Stechmann, Brian Oates, Sergei Nikolaev

Résultat de recherche: Article › examen par les pairs

34 Citations (Scopus)

Résumé

Gliding zooflagellates previously misidentified as Ancyromonas sigmoides, Metopion or Heteromita constitute a new genus Planomonas. Three new Planomonas species (marine P. micra and P. mylnikovi: freshwater P. limna) have extremely divergent 18S rRNA and subtly but consistently different light microscopic morphology, distinguishable from P. (=Ancyromonas) melba comb. nov. and P. (=Bodo) cephalopora comb. nov. Ultrastructurally, P. micra and P. mylnikovi have a sub-plasma membrane dense pellicular layer (except in the ventral feeding pocket whose rim is supported by microtubules), kinetocysts, and flat mitochondrial cristae. Centrioles, connected at ∼80° by short fibres, have a dense amorphous distal plate below a double axosome and four microtubular roots. Microbody, mitochondrion, and dictyosomes associate with the nucleus. Longitudinal cytokinesis is slow and peculiar; ciliary transformation is from anterior to posterior as in other bikonts. Planomonads, like the non-flagellate Micronuclearia (here grouped with planomonads as Hilomonadea cl. nov.), have an indistinguishable single dense pellicular layer, not a double layer like apusomonads (comprising emended class Thecomonadea, phylum Apusozoa). We also sequenced 18S rDNA for Planomonas howeae sp. nov. and Micronuclearia podoventralis, plus actin genes of P. micra, Micronuclearia, Amastigmonas marina. All were analysed phylogenetically; the Planomonas clade is ancient, diverse and robust: it sometimes groups weakly as sister to Micronuclearia.

Langue d'origine	English
Pages (de-à)	535-562
Nombre de pages	28
Journal	Protist
Volume	159
Numéro de publication	4
DOI	https://doi.org/10.1016/j.protis.2008.06.002
Statut de publication	Published - oct. 21 2008
Publié à l'externe	Oui

Note bibliographique

Funding Information:
We thank Alexis Howe and Cédric Berney for isolating the Lake Baikal strains and sequencing their 18S rDNAs and Cédric Berney and Edvard Glücksman for ITS sequencing. We thank A.P. Mylnikov for generously supplying strains and scanning electron micrographs, Lyuba Obolkina for extraordinary help and hospitality at Lake Baikal, and the Institute for Limnology at Irkutsk and many others of its staff for support at Lake Baikal, especially Oleg Timoshkin, Vladimir Obolkin and Nadia Potapskaya. TC-S thanks the Royal Society for a short visit grant, NERC and the Canadian Institute for Advanced Research Evolutionary Biology Program for Fellowship support, and NERC for a research grant. SN thanks Jan Pawlowski for hospitality in his laboratory where he sequenced Micronuclearia and Amastigomonas sp. JJP, supported by the Swiss National Science Foundation.

ASJC Scopus Subject Areas

Microbiology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

ODD de l’ONU

Cette action contribue à la réalisation des objectifs de développement durable suivants

Accès au document

10.1016/j.protis.2008.06.002

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title = "Planomonadida ord. nov. (Apusozoa): Ultrastructural Affinity with Micronuclearia podoventralis and Deep Divergences within Planomonas gen. nov.",

abstract = "Gliding zooflagellates previously misidentified as Ancyromonas sigmoides, Metopion or Heteromita constitute a new genus Planomonas. Three new Planomonas species (marine P. micra and P. mylnikovi: freshwater P. limna) have extremely divergent 18S rRNA and subtly but consistently different light microscopic morphology, distinguishable from P. (=Ancyromonas) melba comb. nov. and P. (=Bodo) cephalopora comb. nov. Ultrastructurally, P. micra and P. mylnikovi have a sub-plasma membrane dense pellicular layer (except in the ventral feeding pocket whose rim is supported by microtubules), kinetocysts, and flat mitochondrial cristae. Centrioles, connected at ∼80° by short fibres, have a dense amorphous distal plate below a double axosome and four microtubular roots. Microbody, mitochondrion, and dictyosomes associate with the nucleus. Longitudinal cytokinesis is slow and peculiar; ciliary transformation is from anterior to posterior as in other bikonts. Planomonads, like the non-flagellate Micronuclearia (here grouped with planomonads as Hilomonadea cl. nov.), have an indistinguishable single dense pellicular layer, not a double layer like apusomonads (comprising emended class Thecomonadea, phylum Apusozoa). We also sequenced 18S rDNA for Planomonas howeae sp. nov. and Micronuclearia podoventralis, plus actin genes of P. micra, Micronuclearia, Amastigmonas marina. All were analysed phylogenetically; the Planomonas clade is ancient, diverse and robust: it sometimes groups weakly as sister to Micronuclearia.",

author = "Thomas Cavalier-Smith and Chao, {Ema E.} and Alexandra Stechmann and Brian Oates and Sergei Nikolaev",

note = "Funding Information: We thank Alexis Howe and C{\'e}dric Berney for isolating the Lake Baikal strains and sequencing their 18S rDNAs and C{\'e}dric Berney and Edvard Gl{\"u}cksman for ITS sequencing. We thank A.P. Mylnikov for generously supplying strains and scanning electron micrographs, Lyuba Obolkina for extraordinary help and hospitality at Lake Baikal, and the Institute for Limnology at Irkutsk and many others of its staff for support at Lake Baikal, especially Oleg Timoshkin, Vladimir Obolkin and Nadia Potapskaya. TC-S thanks the Royal Society for a short visit grant, NERC and the Canadian Institute for Advanced Research Evolutionary Biology Program for Fellowship support, and NERC for a research grant. SN thanks Jan Pawlowski for hospitality in his laboratory where he sequenced Micronuclearia and Amastigomonas sp. JJP, supported by the Swiss National Science Foundation. ",

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doi = "10.1016/j.protis.2008.06.002",

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T2 - Ultrastructural Affinity with Micronuclearia podoventralis and Deep Divergences within Planomonas gen. nov.

AU - Cavalier-Smith, Thomas

AU - Chao, Ema E.

AU - Stechmann, Alexandra

AU - Oates, Brian

AU - Nikolaev, Sergei

N1 - Funding Information: We thank Alexis Howe and Cédric Berney for isolating the Lake Baikal strains and sequencing their 18S rDNAs and Cédric Berney and Edvard Glücksman for ITS sequencing. We thank A.P. Mylnikov for generously supplying strains and scanning electron micrographs, Lyuba Obolkina for extraordinary help and hospitality at Lake Baikal, and the Institute for Limnology at Irkutsk and many others of its staff for support at Lake Baikal, especially Oleg Timoshkin, Vladimir Obolkin and Nadia Potapskaya. TC-S thanks the Royal Society for a short visit grant, NERC and the Canadian Institute for Advanced Research Evolutionary Biology Program for Fellowship support, and NERC for a research grant. SN thanks Jan Pawlowski for hospitality in his laboratory where he sequenced Micronuclearia and Amastigomonas sp. JJP, supported by the Swiss National Science Foundation.

PY - 2008/10/21

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N2 - Gliding zooflagellates previously misidentified as Ancyromonas sigmoides, Metopion or Heteromita constitute a new genus Planomonas. Three new Planomonas species (marine P. micra and P. mylnikovi: freshwater P. limna) have extremely divergent 18S rRNA and subtly but consistently different light microscopic morphology, distinguishable from P. (=Ancyromonas) melba comb. nov. and P. (=Bodo) cephalopora comb. nov. Ultrastructurally, P. micra and P. mylnikovi have a sub-plasma membrane dense pellicular layer (except in the ventral feeding pocket whose rim is supported by microtubules), kinetocysts, and flat mitochondrial cristae. Centrioles, connected at ∼80° by short fibres, have a dense amorphous distal plate below a double axosome and four microtubular roots. Microbody, mitochondrion, and dictyosomes associate with the nucleus. Longitudinal cytokinesis is slow and peculiar; ciliary transformation is from anterior to posterior as in other bikonts. Planomonads, like the non-flagellate Micronuclearia (here grouped with planomonads as Hilomonadea cl. nov.), have an indistinguishable single dense pellicular layer, not a double layer like apusomonads (comprising emended class Thecomonadea, phylum Apusozoa). We also sequenced 18S rDNA for Planomonas howeae sp. nov. and Micronuclearia podoventralis, plus actin genes of P. micra, Micronuclearia, Amastigmonas marina. All were analysed phylogenetically; the Planomonas clade is ancient, diverse and robust: it sometimes groups weakly as sister to Micronuclearia.

AB - Gliding zooflagellates previously misidentified as Ancyromonas sigmoides, Metopion or Heteromita constitute a new genus Planomonas. Three new Planomonas species (marine P. micra and P. mylnikovi: freshwater P. limna) have extremely divergent 18S rRNA and subtly but consistently different light microscopic morphology, distinguishable from P. (=Ancyromonas) melba comb. nov. and P. (=Bodo) cephalopora comb. nov. Ultrastructurally, P. micra and P. mylnikovi have a sub-plasma membrane dense pellicular layer (except in the ventral feeding pocket whose rim is supported by microtubules), kinetocysts, and flat mitochondrial cristae. Centrioles, connected at ∼80° by short fibres, have a dense amorphous distal plate below a double axosome and four microtubular roots. Microbody, mitochondrion, and dictyosomes associate with the nucleus. Longitudinal cytokinesis is slow and peculiar; ciliary transformation is from anterior to posterior as in other bikonts. Planomonads, like the non-flagellate Micronuclearia (here grouped with planomonads as Hilomonadea cl. nov.), have an indistinguishable single dense pellicular layer, not a double layer like apusomonads (comprising emended class Thecomonadea, phylum Apusozoa). We also sequenced 18S rDNA for Planomonas howeae sp. nov. and Micronuclearia podoventralis, plus actin genes of P. micra, Micronuclearia, Amastigmonas marina. All were analysed phylogenetically; the Planomonas clade is ancient, diverse and robust: it sometimes groups weakly as sister to Micronuclearia.

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Planomonadida ord. nov. (Apusozoa): Ultrastructural Affinity with Micronuclearia podoventralis and Deep Divergences within Planomonas gen. nov.

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODD de l’ONU

Accès au document

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