Profiling the small non-coding RNA transcriptome of the human placenta

Victor D. Martinez; David E. Cohn; Nikita Telkar; Brenda C. Minatel; Michelle E. Pewarchuk; Erin A. Marshall; E. Magda Price; Wendy P. Robinson; Wan L. Lam

doi:10.1038/s41597-021-00948-1

Profiling the small non-coding RNA transcriptome of the human placenta

Victor D. Martinez, David E. Cohn, Nikita Telkar, Brenda C. Minatel, Michelle E. Pewarchuk, Erin A. Marshall, E. Magda Price, Wendy P. Robinson, Wan L. Lam

Résultat de recherche: Article › examen par les pairs

8 Citations (Scopus)

Résumé

Proper functioning of the human placenta is critical for maternal and fetal health. While microRNAs (miRNAs) are known to impact placental gene expression, the effects of other small non-coding RNAs (sncRNAs) on the placental transcriptome are not well-established, and are emerging topics in the study of environmental influence on fetal development and reproductive health. Here, we assembled a cohort of 30 placental chorionic villi samples of varying gestational ages (M ± SD = 23.7 ± 11.3 weeks) to delineate the human placental sncRNA transcriptome through small RNA sequence analysis. We observed expression of 1544 sncRNAs, which include 48 miRNAs previously unannotated in humans. Additionally, 18,003 miRNA variants (isomiRs) were identified from the 654 observed miRNA species. This characterization of the term and pre-term placental sncRNA transcriptomes provides data fundamental to future investigations of their regulatory functions in the human placenta, and the baseline expression pattern needed for identifying changes in response to environmental factors, or under disease conditions.

Langue d'origine	English
Numéro d'article	166
Journal	Scientific data
Volume	8
Numéro de publication	1
DOI	https://doi.org/10.1038/s41597-021-00948-1
Statut de publication	Published - déc. 2021
Publié à l'externe	Oui

Note bibliographique

Funding Information:
This work was supported by the National Institutes of Health (R01HD089713) and the Canadian Institutes of Health Research (FDN-143345). E.A.M. is a Vanier Canada Graduate Scholar. The authors thank Dr. Maria Peñaherrera and Ruby Jiang for their assistance with sample processing.

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus Subject Areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

ODD de l’ONU

Cette action contribue à la réalisation des objectifs de développement durable suivants

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10.1038/s41597-021-00948-1

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abstract = "Proper functioning of the human placenta is critical for maternal and fetal health. While microRNAs (miRNAs) are known to impact placental gene expression, the effects of other small non-coding RNAs (sncRNAs) on the placental transcriptome are not well-established, and are emerging topics in the study of environmental influence on fetal development and reproductive health. Here, we assembled a cohort of 30 placental chorionic villi samples of varying gestational ages (M ± SD = 23.7 ± 11.3 weeks) to delineate the human placental sncRNA transcriptome through small RNA sequence analysis. We observed expression of 1544 sncRNAs, which include 48 miRNAs previously unannotated in humans. Additionally, 18,003 miRNA variants (isomiRs) were identified from the 654 observed miRNA species. This characterization of the term and pre-term placental sncRNA transcriptomes provides data fundamental to future investigations of their regulatory functions in the human placenta, and the baseline expression pattern needed for identifying changes in response to environmental factors, or under disease conditions.",

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AU - Marshall, Erin A.

AU - Price, E. Magda

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AU - Lam, Wan L.

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N2 - Proper functioning of the human placenta is critical for maternal and fetal health. While microRNAs (miRNAs) are known to impact placental gene expression, the effects of other small non-coding RNAs (sncRNAs) on the placental transcriptome are not well-established, and are emerging topics in the study of environmental influence on fetal development and reproductive health. Here, we assembled a cohort of 30 placental chorionic villi samples of varying gestational ages (M ± SD = 23.7 ± 11.3 weeks) to delineate the human placental sncRNA transcriptome through small RNA sequence analysis. We observed expression of 1544 sncRNAs, which include 48 miRNAs previously unannotated in humans. Additionally, 18,003 miRNA variants (isomiRs) were identified from the 654 observed miRNA species. This characterization of the term and pre-term placental sncRNA transcriptomes provides data fundamental to future investigations of their regulatory functions in the human placenta, and the baseline expression pattern needed for identifying changes in response to environmental factors, or under disease conditions.

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Profiling the small non-coding RNA transcriptome of the human placenta

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODD de l’ONU

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