Purification of a 3β-hydroxy-Δ⁵-C₂₇-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis

Chemical Compounds

• Acid 9%
• Amino Acid Sequence 24%
• Bile Acid 91%
• Biosynthesis 66%
• Blue 19%
• C19-Steroid 43%
• C21-Steroid 44%
• C27-Steroid 100%
• Chromatography 20%
• Cofactor 24%
• Isoelectric Focusing 29%
• Isoelectric Point 26%
• Protein 10%
• Purification 53%
• SDS Polyacrylamide Gel Electrophoresis 27%
• Sodium Cholate 35%
• Solubilization 25%
• Steroid 24%
• Steroid Hormone 33%

Medicine & Life Sciences

• 2-diethylaminoethanol 13%
• 27-hydroxycholesterol 16%
• 3,7-dihydroxy-5-cholestenoic acid 1%
• 7,25-dihydroxycholesterol 23%
• Amino Acid Sequence 9%
• aminohexyl-sepharose 19%
• Antibodies 5%
• Bile Acids and Salts 82%
• Blue-Sepharose 17%
• Chromatography 9%
• Enzymes 27%
• Hormones 6%
• Hydroxycholesterols 36%
• Isoelectric Focusing 11%
• Isoelectric Point 12%
• Liver Microsomes 91%
• Monoclonal Antibodies 7%
• NAD 10%
• Oxidoreductases 72%
• Polyacrylamide Gel Electrophoresis 9%
• Proteins 3%
• Sepharose 20%
• Sodium Cholate 15%
• Steroids 59%
• Swine 62%