Résumé
The single-electrode voltage-clamp technique requires sharp glass-capillary microelectrodes, whose electrical properties often limit the capabilities of the recording system. Here, we describe a rapid and simple way of coating fine microelectrodes with Dricote and Vaseline that improves their performance during voltage-clamp. The coating prevented clogging of the tips, improved the capacitance compensation of the electrodes, helped to seal the electrode tips into cell membranes and allowed visualization of the tips under saline solution. This new coating method led to greatly improved recordings and better characterization of the transduction and voltage-activated currents in an isolated preparation of spider mechanosensory neurons.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 199-204 |
| Nombre de pages | 6 |
| Journal | Journal of Neuroscience Methods |
| Volume | 71 |
| Numéro de publication | 2 |
| DOI | |
| Statut de publication | Published - févr. 1997 |
Note bibliographique
Funding Information:We thank Hans Polder, Matti Weckström and Ulli Höger for discussions and comments. Special thanks to Steve Shaw for originally suggesting Dricote, to Doug Rasmusson for lending us his Chubbuck-stimulator and to Lynne Maillet-Frotten for assistance with the SEM. Supported by the Medical Research Council of Canada, DFG and NATO.
ASJC Scopus Subject Areas
- General Neuroscience
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't