Résumé
Cells of the yeast Saccharomyces cerevisiae rapidly accumulated in the G1 phase of the cell cycle when exposed to the chelating agents o-phenanthroline (OP) or 8-hydroxyquinoline (HQ). Zinc salts fully reversed the growth-inhibitory effect of both OP and HQ. Cells treated with these chelating agents showed limited RNA accumulation and little RNA degradation. Rates of RNA synthesis were drastically reduced by low concentrations of these compounds, whereas rates of protein synthesis were essentially unaffected. Rates of synthesis of mRNA and tRNA were less affected than were rates of synthesis of high molecular weight RNA. Processing of ribosomal precursor RNA was altered. These results suggest that the primary effect of OP and HQ is on rRNA synthesis. RNA metabolism must therefore have a key role in the regulation of the cell cycle.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 951-958 |
| Nombre de pages | 8 |
| Journal | Cell |
| Volume | 14 |
| Numéro de publication | 4 |
| DOI | |
| Statut de publication | Published - août 1978 |
Note bibliographique
Funding Information:The authors wish to thank D. MacKeigan and C. Ehrhardt for expert technical assistance. This work was supported by a grant from the Medical Research Council of Canada and by the University Internal Medicine Research Foundation. The authors also wish to thank Dr. J. M. Mitchison for a helpful suggestion. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
ASJC Scopus Subject Areas
- General Biochemistry,Genetics and Molecular Biology