Satellites and rapidly reassociating fractions in the DNA of Gadidae (codfishes)

C. W. Helleiner; T. Y. Huh; S. M. Sangster

doi:10.1016/0305-0491(74)90037-6

Satellites and rapidly reassociating fractions in the DNA of Gadidae (codfishes)

C. W. Helleiner, T. Y. Huh, S. M. Sangster

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

1 Citation (Scopus)

Résumé

1. 1. The DNA of all six species of Gadidae (cofishes) investigated contained at least one satellite component, revealed by isopycnic centrifugation either in CsCl or in Cs₂SO₄ solutions containing Ag⁺ or Hg²⁺. 2. 2. The unfractionated DNA of three species investigated in this regard contained 30-40 per cent of a rapidly reassociating fraction (C₀t less than 0·1 mole.sec per 1.). The DNA of all six species contained a fraction which banded as reassociated DNA after heating and cooling. 3. 3. One of the satellite fractions of Atlantic cod DNA was isolated. Its kinetic complexity suggests a repeating unit of 4400 base pairs, represented 7000 times in the genome.

Langue d'origine	English
Pages (de-à)	11-24
Nombre de pages	14
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	48
Numéro de publication	1
DOI	https://doi.org/10.1016/0305-0491(74)90037-6
Statut de publication	Published - mai 15 1974

Note bibliographique

Funding Information:
work was supported by grants from the Medical Research Council of Canada.

ASJC Scopus Subject Areas

Biochemistry
Physiology
Molecular Biology

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10.1016/0305-0491(74)90037-6

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abstract = "1. 1. The DNA of all six species of Gadidae (cofishes) investigated contained at least one satellite component, revealed by isopycnic centrifugation either in CsCl or in Cs2SO4 solutions containing Ag+ or Hg2+. 2. 2. The unfractionated DNA of three species investigated in this regard contained 30-40 per cent of a rapidly reassociating fraction (C0t less than 0·1 mole.sec per 1.). The DNA of all six species contained a fraction which banded as reassociated DNA after heating and cooling. 3. 3. One of the satellite fractions of Atlantic cod DNA was isolated. Its kinetic complexity suggests a repeating unit of 4400 base pairs, represented 7000 times in the genome.",

author = "Helleiner, {C. W.} and Huh, {T. Y.} and Sangster, {S. M.}",

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AU - Huh, T. Y.

AU - Sangster, S. M.

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AB - 1. 1. The DNA of all six species of Gadidae (cofishes) investigated contained at least one satellite component, revealed by isopycnic centrifugation either in CsCl or in Cs2SO4 solutions containing Ag+ or Hg2+. 2. 2. The unfractionated DNA of three species investigated in this regard contained 30-40 per cent of a rapidly reassociating fraction (C0t less than 0·1 mole.sec per 1.). The DNA of all six species contained a fraction which banded as reassociated DNA after heating and cooling. 3. 3. One of the satellite fractions of Atlantic cod DNA was isolated. Its kinetic complexity suggests a repeating unit of 4400 base pairs, represented 7000 times in the genome.

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Satellites and rapidly reassociating fractions in the DNA of Gadidae (codfishes)

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

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