Résumé
The genome segment B sequence of infectious pancreatic necrosis virus was determined for both the Jasper and Sp serotypes. The sequences are 2784 and 2630 by long, respectively, and contain a single large open reading frame encoding the VP1 protein, the putative RNA-dependent RNA polymerase (RdRp) of IPNV. The proteins exhibit an 88% homology with each other, but only 41% with infectious bursal disease virus (IBDV) VP1, another member of the Birnaviridae. Despite the low overall homology between the IPNV and IBDV VP1 proteins, homologous regions were detected within the central portion of the proteins. The carboxy-proximal regions of the VP1, which contain very low amino acid homology, displayed evidence of conservation in structural features such as a hydrophilic, highly basic domain. Consensus sequences associated with GTP-binding proteins and RdRps were also detected in VP1. However, unlike the RdRps associated with single-stranded plus RNA viruses, the birnavirus RdRp lacks the Gly-Asp-Asp motif characteristic of this enzyme family.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 541-552 |
| Nombre de pages | 12 |
| Journal | Virology |
| Volume | 181 |
| Numéro de publication | 2 |
| DOI | |
| Statut de publication | Published - avr. 1991 |
| Publié à l'externe | Oui |
Note bibliographique
Funding Information:Work performed at the Universrty of Guelph was supported by the Natural Sciences and Engineering Research Council of Canada. R.D. IS an Alberta Heritage Foundation for Medical Research Postdoctoral Fellow. Portions of this work were performed in Dr. Patrick Lee’s laboratory at the University of Calgary. The work performed at Oregon State University was supported by Oregon Sea Grant with funds from the National Oceanic and Atmospheric Administration, Office of Sea Grant, Department of Commerce, under Grants NA81 AA-D-00086 (Project R/FSD-9) and NA85AA-DSG095 (Project R/FSD-1 1 and R/FSD-13) and from appropriations made the Oregon State Legislature.
ASJC Scopus Subject Areas
- Virology