Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets

Magen E. Francis; Brian Richardson; Una Goncin; Mara McNeil; Melissa Rioux; Mary K. Foley; Anni Ge; Roger D. Pechous; Jason Kindrachuk; Cheryl M. Cameron; Christopher Richardson; Jocelyne Lew; Steven Machtaler; Mark J. Cameron; Volker Gerdts; Darryl Falzarano; Alyson A. Kelvin

doi:10.1038/s41598-021-93855-9

Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets

Magen E. Francis, Brian Richardson, Una Goncin, Mara McNeil, Melissa Rioux, Mary K. Foley, Anni Ge, Roger D. Pechous, Jason Kindrachuk, Cheryl M. Cameron, Christopher Richardson, Jocelyne Lew, Steven Machtaler, Mark J. Cameron, Volker Gerdts, Darryl Falzarano, Alyson A. Kelvin

Medicine

Résultat de recherche: Article › examen par les pairs

13 Citations (Scopus)

Résumé

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) hospitalizations and deaths disportionally affect males and older ages. Here we investigated the impact of male sex and age comparing sex-matched or age-matched ferrets infected with SARS-CoV-2. Differences in temperature regulation was identified for male ferrets which was accompanied by prolonged viral replication in the upper respiratory tract after infection. Gene expression analysis of the nasal turbinates indicated that 1-year-old female ferrets had significant increases in interferon response genes post infection which were delayed in males. These results provide insight into COVID-19 and suggests that older males may play a role in viral transmission due to decreased antiviral responses.

Langue d'origine	English
Numéro d'article	14536
Journal	Scientific Reports
Volume	11
Numéro de publication	1
DOI	https://doi.org/10.1038/s41598-021-93855-9
Statut de publication	Published - déc. 2021

Note bibliographique

Funding Information:
This study was also funded by IWK Health Centre, mitacs, Natural Sciences and Engineering Research Council of Canada, National Institute of Allergy and Infectious Diseases, CFI and The Canadian Institutes for Health Research (CIHR).

Funding Information:
A. Kelvin is funded by the Canadian 2019 Novel Coronavirus (COVID-19) Rapid Research Funding initiative the Canadian Institutes of Health Research (CIHR) (Grant numbers OV5-170349, VRI-172779, and OV2-170357) and Atlantic Genome/Genome Canada, Scotiabank COVID-19 IMPACT Grant, and the Nova Scotia COVID-19 Health Research Coalition. D. Falzarano is funded by the Canadian Institutes of Health Research (CIHR), Grant number OV5-170349. M. Cameron, C. Cameron, and B. Richardson are supported by NIH/ NIAID (3R01AI129709-03S1) and the Nord Family Foundation, Amherst, Ohio. R. D. Pechous is supported by NIH/NIAID (award R56AI153252). J. Kindrachuk is funded by a Tier 2 Canada Research Chair in the Molecular Pathogenesis of Emerging and Re-Emerging Viruses provided by the Canadian Institutes of Health Research (Grant no. 950-231498). C. Richardson is funded by a CIHR COVID-19 Rapid Funding Opportunity VR1-172779. The authors would like to thank the tremendous efforts of the VIDO-InterVac veterinary staff including Dr. Colette Wheler. We also thank the staff of the CWRU Applied Functional Genomics Core for performing the RNA-Sequencing assays. Operational support at VIDO-InterVac is provided in part by the Canadian Foundation for Innovation through the Major Science Initiates Fund and by Innovation Saskatchewan. This article is published with the permission of the Director of VIDO-InterVac.

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus Subject Areas

General

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

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10.1038/s41598-021-93855-9

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Francis, M. E., Richardson, B., Goncin, U., McNeil, M., Rioux, M., Foley, M. K., Ge, A., Pechous, R. D., Kindrachuk, J., Cameron, C. M., Richardson, C., Lew, J., Machtaler, S., Cameron, M. J., Gerdts, V., Falzarano, D., & Kelvin, A. A. (2021). Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets. Scientific Reports, 11(1), Article 14536. https://doi.org/10.1038/s41598-021-93855-9

Francis, ME, Richardson, B, Goncin, U, McNeil, M, Rioux, M, Foley, MK, Ge, A, Pechous, RD, Kindrachuk, J, Cameron, CM, Richardson, C, Lew, J, Machtaler, S, Cameron, MJ, Gerdts, V, Falzarano, D & Kelvin, AA 2021, 'Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets', Scientific Reports, vol. 11, n° 1, 14536. https://doi.org/10.1038/s41598-021-93855-9

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title = "Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets",

abstract = "SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) hospitalizations and deaths disportionally affect males and older ages. Here we investigated the impact of male sex and age comparing sex-matched or age-matched ferrets infected with SARS-CoV-2. Differences in temperature regulation was identified for male ferrets which was accompanied by prolonged viral replication in the upper respiratory tract after infection. Gene expression analysis of the nasal turbinates indicated that 1-year-old female ferrets had significant increases in interferon response genes post infection which were delayed in males. These results provide insight into COVID-19 and suggests that older males may play a role in viral transmission due to decreased antiviral responses.",

author = "Francis, {Magen E.} and Brian Richardson and Una Goncin and Mara McNeil and Melissa Rioux and Foley, {Mary K.} and Anni Ge and Pechous, {Roger D.} and Jason Kindrachuk and Cameron, {Cheryl M.} and Christopher Richardson and Jocelyne Lew and Steven Machtaler and Cameron, {Mark J.} and Volker Gerdts and Darryl Falzarano and Kelvin, {Alyson A.}",

note = "Funding Information: This study was also funded by IWK Health Centre, mitacs, Natural Sciences and Engineering Research Council of Canada, National Institute of Allergy and Infectious Diseases, CFI and The Canadian Institutes for Health Research (CIHR). Funding Information: A. Kelvin is funded by the Canadian 2019 Novel Coronavirus (COVID-19) Rapid Research Funding initiative the Canadian Institutes of Health Research (CIHR) (Grant numbers OV5-170349, VRI-172779, and OV2-170357) and Atlantic Genome/Genome Canada, Scotiabank COVID-19 IMPACT Grant, and the Nova Scotia COVID-19 Health Research Coalition. D. Falzarano is funded by the Canadian Institutes of Health Research (CIHR), Grant number OV5-170349. M. Cameron, C. Cameron, and B. Richardson are supported by NIH/ NIAID (3R01AI129709-03S1) and the Nord Family Foundation, Amherst, Ohio. R. D. Pechous is supported by NIH/NIAID (award R56AI153252). J. Kindrachuk is funded by a Tier 2 Canada Research Chair in the Molecular Pathogenesis of Emerging and Re-Emerging Viruses provided by the Canadian Institutes of Health Research (Grant no. 950-231498). C. Richardson is funded by a CIHR COVID-19 Rapid Funding Opportunity VR1-172779. The authors would like to thank the tremendous efforts of the VIDO-InterVac veterinary staff including Dr. Colette Wheler. We also thank the staff of the CWRU Applied Functional Genomics Core for performing the RNA-Sequencing assays. Operational support at VIDO-InterVac is provided in part by the Canadian Foundation for Innovation through the Major Science Initiates Fund and by Innovation Saskatchewan. This article is published with the permission of the Director of VIDO-InterVac. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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doi = "10.1038/s41598-021-93855-9",

language = "English",

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AU - Francis, Magen E.

AU - Richardson, Brian

AU - Goncin, Una

AU - McNeil, Mara

AU - Rioux, Melissa

AU - Foley, Mary K.

AU - Ge, Anni

AU - Pechous, Roger D.

AU - Kindrachuk, Jason

AU - Cameron, Cheryl M.

AU - Richardson, Christopher

AU - Lew, Jocelyne

AU - Machtaler, Steven

AU - Cameron, Mark J.

AU - Gerdts, Volker

AU - Falzarano, Darryl

AU - Kelvin, Alyson A.

N1 - Funding Information: This study was also funded by IWK Health Centre, mitacs, Natural Sciences and Engineering Research Council of Canada, National Institute of Allergy and Infectious Diseases, CFI and The Canadian Institutes for Health Research (CIHR). Funding Information: A. Kelvin is funded by the Canadian 2019 Novel Coronavirus (COVID-19) Rapid Research Funding initiative the Canadian Institutes of Health Research (CIHR) (Grant numbers OV5-170349, VRI-172779, and OV2-170357) and Atlantic Genome/Genome Canada, Scotiabank COVID-19 IMPACT Grant, and the Nova Scotia COVID-19 Health Research Coalition. D. Falzarano is funded by the Canadian Institutes of Health Research (CIHR), Grant number OV5-170349. M. Cameron, C. Cameron, and B. Richardson are supported by NIH/ NIAID (3R01AI129709-03S1) and the Nord Family Foundation, Amherst, Ohio. R. D. Pechous is supported by NIH/NIAID (award R56AI153252). J. Kindrachuk is funded by a Tier 2 Canada Research Chair in the Molecular Pathogenesis of Emerging and Re-Emerging Viruses provided by the Canadian Institutes of Health Research (Grant no. 950-231498). C. Richardson is funded by a CIHR COVID-19 Rapid Funding Opportunity VR1-172779. The authors would like to thank the tremendous efforts of the VIDO-InterVac veterinary staff including Dr. Colette Wheler. We also thank the staff of the CWRU Applied Functional Genomics Core for performing the RNA-Sequencing assays. Operational support at VIDO-InterVac is provided in part by the Canadian Foundation for Innovation through the Major Science Initiates Fund and by Innovation Saskatchewan. This article is published with the permission of the Director of VIDO-InterVac. Publisher Copyright: © 2021, The Author(s).

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AB - SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) hospitalizations and deaths disportionally affect males and older ages. Here we investigated the impact of male sex and age comparing sex-matched or age-matched ferrets infected with SARS-CoV-2. Differences in temperature regulation was identified for male ferrets which was accompanied by prolonged viral replication in the upper respiratory tract after infection. Gene expression analysis of the nasal turbinates indicated that 1-year-old female ferrets had significant increases in interferon response genes post infection which were delayed in males. These results provide insight into COVID-19 and suggests that older males may play a role in viral transmission due to decreased antiviral responses.

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Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Accès au document

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