Résumé
In contrast to earlier proposals, recent evidence suggests that trans- spliceosomes in trypanosomatid protozoa may contain a homolog of U1 small nuclear (sn) RNA (Schnare, M.N. and Gray, M.W. (1999) J. Biol. Chem. 274, 23 691-23 694). However, the candidate trypanosomatid U1 snRNA is unconventional because it lacks the highly conserved stem/loop II present in all other U1 snRNAs. Trypanosomatids also possess a unique spliced leader-associated (SLA) RNA of unknown function. We present the complete sequence of the SLA RNA from Crithidia fasciculata and propose that it may contribute a U1 snRNA-like stem/loop II to the trans-spliceosome.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 215-217 |
| Nombre de pages | 3 |
| Journal | FEBS Letters |
| Volume | 459 |
| Numéro de publication | 2 |
| DOI | |
| Statut de publication | Published - oct. 8 1999 |
Note bibliographique
Funding Information:We thank R. Breckon and F.B.St.C. Palmer for cultures of C. fasciculata , D.F. Spencer for C. fasciculata DNA, M. McManus and S.L. Hajduk for T. brucei RNA and Y. Watanabe and D.F. Spencer for technical advice. We also thank B. Weiser and H. Noller for the computer program XRNA, which was used to generate the secondary structure figure. A Grant (MT-11212) to M.W.G. from the Medical Research Council of Canada and salary support from the Canadian Institute for Advanced Research (Program in Evolutionary Biology) are gratefully acknowledged.
ASJC Scopus Subject Areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology