Résumé
U5 snRNAs in trypanosomatid protozoa do not contain the trimethylguanosine cap structures that are often targeted in snRNA isolation procedures. As a result, the trypanosomatids are not well represented in the database of available U5 snRNA sequences. We have isolated and determined the sequence of the U5 snRNA from Crithidia fasciculata. Comparison with previously published trypanosomatid U5 snRNA sequences allows us to deduce the pattern of structural conservation and variation among these very divergent snRNA molecules. (C) 2000 Elsevier Science B.V.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 362-366 |
| Nombre de pages | 5 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1490 |
| Numéro de publication | 3 |
| DOI | |
| Statut de publication | Published - févr. 29 2000 |
Note bibliographique
Funding Information:We thank F.B.St.C. Palmer and R. Breckon for supplying cultures of C. fasciculata, and D.F. Spencer for a gift of purified C. fasciculata DNA and for providing the chemical sequencing protocol. Grant support to M.W.G. from the Medical Research Council of Canada (operating grant MT-11212) and salary support in the form of a fellowship from the Canadian Institute for Advanced Research (Program in Evolutionary Biology) are gratefully acknowledged.
ASJC Scopus Subject Areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics