Studying trophic ecology in marine ecosystems using fatty acids: A primer on analysis and interpretation

FA signature analysis has the potential to be a very powerful technique in both qualitative and quantitative investigations in many areas of ecology, particularly in trophic studies. In this review, we have attempted to provide both general guidelines, as well as detailed procedures, to follow for accurate analysis while avoiding some common pitfalls. The underlying message is, in all cases, to use common sense and prudence in sample collection, preparation, analysis, and interpretation. Some understanding of the protocols described here will allow biologists to make logical decisions regarding the course to follow in collecting and analyzing samples and in interpreting resulting data.