Subcellular location of Piscirickettsia salmonis heat shock protein 60 (Hsp60) chaperone by using immunogold labeling and proteomic analysis

Cristian Oliver, Patricio Sánchez, Karla Valenzuela, Mauricio Hernández, Juan Pablo Pontigo, Maria C. Rauch, Rafael A. Garduño, Ruben Avendaño-Herrera, Alejandro J. Yáñez

Résultat de recherche: Articleexamen par les pairs

4 Citations (Scopus)

Résumé

Piscirickettsia salmonis is the causative bacterial agent of piscirickettsiosis, a systemic fish disease that significantly impacts the Chilean salmon industry. This bacterium possesses a type IV secretion system (T4SS), several proteins of the type III secretion system (T3SS), and a single heat shock protein 60 (Hsp60/GroEL). It has been suggested that due to its high antigenicity, the P. salmonis Hsp60 could be surface-exposed, translocated across the membrane, and (or) secreted into the extracellular matrix. This study tests the hypothesis that P. salmonis Hsp60 could be located on the bacterial surface. Immunogold electron microscopy and proteomic analyses suggested that although P. salmonis Hsp60 was predominantly associated with the bacterial cell cytoplasm, Hsp60-positive spots also exist on the bacterial cell envelope. IgY antibodies against P. salmonis Hsp60 protected SHK-1 cells against infection. Several bioinformatics approaches were used to assess Hsp60 translocation by the T4SS, T3SS, and T6SS, with negative results. These data support the hypothesis that small amounts of Hsp60 must reach the bacterial cell surface in a manner probably not mediated by currently characterized secretion systems, and that they remain biologically active during P. salmonis infection, possibly mediating adherence and (or) invasion.

Langue d'origineEnglish
Numéro d'article117
JournalMicroorganisms
Volume8
Numéro de publication1
DOI
Statut de publicationPublished - janv. 2020

Note bibliographique

Funding Information:
Funding: This work was supported by the Comisión Nacional de Investigación Científica y Tecnológica (Grant numbers FONDAP INCAR N°15110027, FONDEF VIU15E0006 and FONDECYT Iniciación N°11180994) and by the Vicerrectoría de Investigación, Desarrollo y Creación Artística (VIDCA) of the Universidad Austral de Chile.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

ASJC Scopus Subject Areas

  • Microbiology
  • Microbiology (medical)
  • Virology

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