The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart

Konrad Meissner; Björn Heydrich; Gabriele Jedlitschky; Henriette Meyer Zu Schwabedissen; Igor Mosyagin; Peter Dazert; Lothar Eckel; Silke Vogelgesang; Rolf W. Warzok; Michael Böhm; Christian Lehmann; Michael Wendt; Ingolf Cascorbi; Heyo K. Kroemer

doi:10.1369/jhc.5A6750.2005

The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart

Konrad Meissner, Björn Heydrich, Gabriele Jedlitschky, Henriette Meyer Zu Schwabedissen, Igor Mosyagin, Peter Dazert, Lothar Eckel, Silke Vogelgesang, Rolf W. Warzok, Michael Böhm, Christian Lehmann, Michael Wendt, Ingolf Cascorbi, Heyo K. Kroemer

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Résumé

Efforts to improve severely impaired myocardial function include transplantation of autologous hematopoietic side population (SP) stem cells. The transmembrane ABC-type (ATP binding cassette) half-transporter ABCG2 (BCRP) serves as a marker protein for SP cell selection. We have recently shown that other ABC transport proteins such as ABCB1 and ABCC5 are differentially expressed in normal and diseased human heart. Here we investigated localization and individual ABCG2 expression in 15 ventricular (including 10 cardiomyopathic) and 51 auricular heart tissue samples using immunohistochemistry, confocal laser scanning fluorescence microscopy, and real-time RT-PCR. Individual genotypes were assigned using PCR-restriction fragment length polymorphism (RFLP) analysis and subsequently correlated to ABCG2 mRNA levels. ABCG2 was localized in endothelial cells of capillaries and arterioles of all samples. Ventricular samples from cardiomyopathic hearts exhibited significantly increased levels of ABCG2 mRNA (ABCG2/18S rRNA: 1.08 ± 0.30 × 10^-7; p=0.028 (dilative cardiomyopathy) and 1.16 ± 0.46 × 10^-7; p=0.009 (ischemic cardiomyopathy) compared with 0.44 ± 0.26 × 10^-7 in nonfailing hearts). The individual haplotypes were not associated with altered mRNA expression. ABCG2 is variably expressed in endothelial cells of human heart, where it may function as a protective barrier against cardiotoxic drugs such as anthracydines or mitoxantrone. ABCG2 expression is induced in dilative and ischemic cardiomyopathies.

Langue d'origine	English
Pages (de-à)	215-221
Nombre de pages	7
Journal	Journal of Histochemistry and Cytochemistry
Volume	54
Numéro de publication	2
DOI	https://doi.org/10.1369/jhc.5A6750.2005
Statut de publication	Published - févr. 2006
Publié à l'externe	Oui

ASJC Scopus Subject Areas

Anatomy
Histology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

ODD de l’ONU

Cette action contribue à la réalisation des objectifs de développement durable suivants

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10.1369/jhc.5A6750.2005

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Meissner, K., Heydrich, B., Jedlitschky, G., Meyer Zu Schwabedissen, H., Mosyagin, I., Dazert, P., Eckel, L., Vogelgesang, S., Warzok, R. W., Böhm, M., Lehmann, C., Wendt, M., Cascorbi, I., & Kroemer, H. K. (2006). The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart. Journal of Histochemistry and Cytochemistry, 54(2), 215-221. https://doi.org/10.1369/jhc.5A6750.2005

Meissner, K, Heydrich, B, Jedlitschky, G, Meyer Zu Schwabedissen, H, Mosyagin, I, Dazert, P, Eckel, L, Vogelgesang, S, Warzok, RW, Böhm, M, Lehmann, C, Wendt, M, Cascorbi, I & Kroemer, HK 2006, 'The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart', Journal of Histochemistry and Cytochemistry, vol. 54, n° 2, pp. 215-221. https://doi.org/10.1369/jhc.5A6750.2005

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title = "The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart",

abstract = "Efforts to improve severely impaired myocardial function include transplantation of autologous hematopoietic side population (SP) stem cells. The transmembrane ABC-type (ATP binding cassette) half-transporter ABCG2 (BCRP) serves as a marker protein for SP cell selection. We have recently shown that other ABC transport proteins such as ABCB1 and ABCC5 are differentially expressed in normal and diseased human heart. Here we investigated localization and individual ABCG2 expression in 15 ventricular (including 10 cardiomyopathic) and 51 auricular heart tissue samples using immunohistochemistry, confocal laser scanning fluorescence microscopy, and real-time RT-PCR. Individual genotypes were assigned using PCR-restriction fragment length polymorphism (RFLP) analysis and subsequently correlated to ABCG2 mRNA levels. ABCG2 was localized in endothelial cells of capillaries and arterioles of all samples. Ventricular samples from cardiomyopathic hearts exhibited significantly increased levels of ABCG2 mRNA (ABCG2/18S rRNA: 1.08 ± 0.30 × 10-7; p=0.028 (dilative cardiomyopathy) and 1.16 ± 0.46 × 10-7; p=0.009 (ischemic cardiomyopathy) compared with 0.44 ± 0.26 × 10-7 in nonfailing hearts). The individual haplotypes were not associated with altered mRNA expression. ABCG2 is variably expressed in endothelial cells of human heart, where it may function as a protective barrier against cardiotoxic drugs such as anthracydines or mitoxantrone. ABCG2 expression is induced in dilative and ischemic cardiomyopathies.",

author = "Konrad Meissner and Bj{\"o}rn Heydrich and Gabriele Jedlitschky and {Meyer Zu Schwabedissen}, Henriette and Igor Mosyagin and Peter Dazert and Lothar Eckel and Silke Vogelgesang and Warzok, {Rolf W.} and Michael B{\"o}hm and Christian Lehmann and Michael Wendt and Ingolf Cascorbi and Kroemer, {Heyo K.}",

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doi = "10.1369/jhc.5A6750.2005",

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AU - Meissner, Konrad

AU - Heydrich, Björn

AU - Jedlitschky, Gabriele

AU - Meyer Zu Schwabedissen, Henriette

AU - Mosyagin, Igor

AU - Dazert, Peter

AU - Eckel, Lothar

AU - Vogelgesang, Silke

AU - Warzok, Rolf W.

AU - Böhm, Michael

AU - Lehmann, Christian

AU - Wendt, Michael

AU - Cascorbi, Ingolf

AU - Kroemer, Heyo K.

PY - 2006/2

Y1 - 2006/2

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AB - Efforts to improve severely impaired myocardial function include transplantation of autologous hematopoietic side population (SP) stem cells. The transmembrane ABC-type (ATP binding cassette) half-transporter ABCG2 (BCRP) serves as a marker protein for SP cell selection. We have recently shown that other ABC transport proteins such as ABCB1 and ABCC5 are differentially expressed in normal and diseased human heart. Here we investigated localization and individual ABCG2 expression in 15 ventricular (including 10 cardiomyopathic) and 51 auricular heart tissue samples using immunohistochemistry, confocal laser scanning fluorescence microscopy, and real-time RT-PCR. Individual genotypes were assigned using PCR-restriction fragment length polymorphism (RFLP) analysis and subsequently correlated to ABCG2 mRNA levels. ABCG2 was localized in endothelial cells of capillaries and arterioles of all samples. Ventricular samples from cardiomyopathic hearts exhibited significantly increased levels of ABCG2 mRNA (ABCG2/18S rRNA: 1.08 ± 0.30 × 10-7; p=0.028 (dilative cardiomyopathy) and 1.16 ± 0.46 × 10-7; p=0.009 (ischemic cardiomyopathy) compared with 0.44 ± 0.26 × 10-7 in nonfailing hearts). The individual haplotypes were not associated with altered mRNA expression. ABCG2 is variably expressed in endothelial cells of human heart, where it may function as a protective barrier against cardiotoxic drugs such as anthracydines or mitoxantrone. ABCG2 expression is induced in dilative and ischemic cardiomyopathies.

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ER -

The ATP-binding cassette transporter ABCG2 (BCRP), a marker for side population stem cells, is expressed in human heart

Résumé

ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODD de l’ONU

Accès au document

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