The measurement of theophylline metabolism in hepatic microsomes using high‐performance liquid chromatography

Theresa C. Hemsworth; Kenneth W. Renton

doi:10.1002/jps.2600710628

The measurement of theophylline metabolism in hepatic microsomes using high‐performance liquid chromatography

Theresa C. Hemsworth, Kenneth W. Renton

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

4 Citations (Scopus)

Résumé

A method is described to measure the in vitro metabolism of theophylline by liver microsomes. The formation of 1‐methyluric acid and 1, 3‐dimethyluric acid in incubation mixtures was determined by high‐performance liquid chromatography. The formation of both metabolites was linear with time and the formation of 1‐methyluric acid was blocked by allopurinol. This method will be useful in assessing potential drug interactions involving theophylline.

Langue d'origine	English
Pages (de-à)	712-713
Nombre de pages	2
Journal	Journal of Pharmaceutical Sciences
Volume	71
Numéro de publication	6
DOI	https://doi.org/10.1002/jps.2600710628
Statut de publication	Published - juin 1982

Note bibliographique

Funding Information:
Supported by the Medical Research Council of Canada

ASJC Scopus Subject Areas

Pharmaceutical Science

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10.1002/jps.2600710628

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abstract = "A method is described to measure the in vitro metabolism of theophylline by liver microsomes. The formation of 1‐methyluric acid and 1, 3‐dimethyluric acid in incubation mixtures was determined by high‐performance liquid chromatography. The formation of both metabolites was linear with time and the formation of 1‐methyluric acid was blocked by allopurinol. This method will be useful in assessing potential drug interactions involving theophylline.",

author = "Hemsworth, {Theresa C.} and Renton, {Kenneth W.}",

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AB - A method is described to measure the in vitro metabolism of theophylline by liver microsomes. The formation of 1‐methyluric acid and 1, 3‐dimethyluric acid in incubation mixtures was determined by high‐performance liquid chromatography. The formation of both metabolites was linear with time and the formation of 1‐methyluric acid was blocked by allopurinol. This method will be useful in assessing potential drug interactions involving theophylline.

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The measurement of theophylline metabolism in hepatic microsomes using high‐performance liquid chromatography

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

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