Résumé
Premise: Lace plant (Aponogeton madagascariensis) leaves are remodeled via developmental programmed cell death (PCD) to produce perforations located equidistantly between longitudinal and transverse veins. Auxin has been implicated in other developmental PCD processes in plants; however, the role of auxin in perforation formation in lace plant is unknown. Here the role of auxin in developmental PCD in lace plant was studied using two auxin inhibitors N-1-naphthylphthalamic acid (NPA), an auxin transport inhibitor, and auxinole, a potent auxin antagonist. Methods: Sterile cultures of lace plants were propagated and treated with NPA or auxinole. Leaf length, leaf width, and number of perforations were then analyzed. Vein patterning and perforation area were further examined in NPA-treated plants. Downstream PCD transduction events were investigated via spectrophotometric assays, histochemical staining, and immuno-probing. Results: Lace plants treated with NPA or auxinole produced leaves with fewer perforations compared to their respective controls. Although NPA treatment was insufficient to completely alter vein patterning, NPA-treated leaves did have significantly more atypical areoles compared to control leaves. Events involved in perforation formation in lace plant leaves were altered following treatment with NPA, including anthocyanin production, reactive oxygen species (ROS) accumulation, and the release of mitochondrial cytochrome c. Conclusions: Our results indicated that inhibition of auxin signaling disrupts several downstream features of the lace plant PCD signaling cascade and results in fewer or no perforations. Therefore, we concluded that auxin signaling is important for developmentally regulated PCD in lace plant leaves.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 577-586 |
| Nombre de pages | 10 |
| Journal | American Journal of Botany |
| Volume | 107 |
| Numéro de publication | 4 |
| DOI | |
| Statut de publication | Published - avr. 1 2020 |
Note bibliographique
Funding Information:We greatly acknowledge Dr. Enrico Scarpella's (Department of Biological Sciences, University of Alberta, Canada) guidance throughout the project. Thank you to Dr. Ken‐Ichiro Hayashi, Okayama University of Science, Okayama, Japan for providing auxinole. We also thank the reviewers for their valuable feedback on the manuscript. We thank the Natural Sciences and Engineering Research Council of Canada for Discovery and Accelerator Supplement Grants to A.H.L.A.N.G. A.N.D. was supported by NSERC Discovery Accelerator Supplements (DAS) to A.H.L.A.N.G. G.L.D. is currently supported by a Nova Scotia Research and Innovation Graduate Scholarship, NSERC Canadian Graduate Scholarship and A.H.L.A.N.G's Discovery Grant (#2017‐04299). We also acknowledge the Sarah Lawson Research Scholarship (Dalhousie University) and NSERC USRA for funding for M.S.F. and G.L.D.
Funding Information:
We greatly acknowledge Dr. Enrico Scarpella's (Department of Biological Sciences, University of Alberta, Canada) guidance throughout the project. Thank you to Dr. Ken-Ichiro Hayashi, Okayama University of Science, Okayama, Japan for providing auxinole. We also thank the reviewers for their valuable feedback on the manuscript. We thank the Natural Sciences and Engineering Research Council of Canada for Discovery and Accelerator Supplement Grants to A.H.L.A.N.G. A.N.D. was supported by NSERC Discovery Accelerator Supplements (DAS) to A.H.L.A.N.G. G.L.D. is currently supported by a Nova Scotia Research and Innovation Graduate Scholarship, NSERC Canadian Graduate Scholarship and A.H.L.A.N.G's Discovery Grant (#2017-04299). We also acknowledge the Sarah Lawson Research Scholarship (Dalhousie University) and NSERC USRA for funding for M.S.F. and G.L.D.
Publisher Copyright:
© 2020 Botanical Society of America
ASJC Scopus Subject Areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Plant Science
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't