Résumé
Background: Mechanical stimuli imparted by skeletal muscles play an important role during embryonic development in vertebrates. Little is known whether skeletal muscles are required for normal external ear development. Methods: We used Myf5-/-:MyoD-/- (double-mutant) mouse embryos that completely lack skeletal musculature and analyzed the development of the external ear. We concentrated on the external ear because several studies have suggested a muscular cause to various congenital auricular deformities, and middle and inner ear development was previously reported using the same mouse model. Wild-type mouse embryos were used as controls to compare the histomorphometric outcomes. Results: Our findings demonstrated an absence of the external auditory meatus, along with an abnormal auricular appearance, in the double-mutant mouse embryos. Specifically, the auricle did not protrude laterally as noted in the wild-type mouse ears. However, histomorphometric measurements were not significantly different between the wild-type and doublemutant mouse ears. Conclusion: Overall, our study showed that the development of the mouse external ear is dependent on the presence of skeletal muscles.
| Langue d'origine | English |
|---|---|
| Numéro d'article | e382 |
| Journal | Plastic and Reconstructive Surgery - Global Open |
| Volume | 3 |
| Numéro de publication | 5 |
| DOI | |
| Statut de publication | Published - 2015 |
Note bibliographique
Funding Information:Disclosure: Dr. Kablar received an infrastructure grant from the Canada Foundation for Innovation. Neither of the other authors has any financial disclosures. This study was supported by an operating grant from the Natural Sciences and Engineering Research Council of Canada (Grant Number 238726-01). The Article Processing Charge was paid for by the authors.
Publisher Copyright:
Copyright © 2015 The Authors.
ASJC Scopus Subject Areas
- Surgery