tRNA conjugation with chitosan nanoparticles: An AFM imaging study

D. Agudelo; L. Kreplak; H. A. Tajmir-Riahi

doi:10.1016/j.ijbiomac.2015.12.057

tRNA conjugation with chitosan nanoparticles: An AFM imaging study

D. Agudelo, L. Kreplak, H. A. Tajmir-Riahi

Résultat de recherche: Article › examen par les pairs

13 Citations (Scopus)

Résumé

The conjugation of tRNA with chitosan nanoparticles of different sizes 15,100 and 200kDa was investigated in aqueous solution using multiple spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed that chitosan binds tRNA via G-C and A-U base pairs as well as backbone PO₂ group, through electrostatic, hydrophilic and H-bonding contacts with overall binding constants of K_Ch-15-tRNA=4.1 (±0.60)×10³M^-1, K_Ch-100-tRNA=5.7 (±0.8)×10³M^-1 and K_Ch-200-tRNA=1.2 (±0.3)×10⁴M^-1. As chitosan size increases more stable polymer-tRNA conjugate is formed. AFM images showed major tRNA aggregation and particle formation occurred as chitosan concentration increased. Even though chitosan induced major biopolymer structural changes, tRNA remains in A-family structure.

Langue d'origine	English
Pages (de-à)	150-156
Nombre de pages	7
Journal	International Journal of Biological Macromolecules
Volume	85
DOI	https://doi.org/10.1016/j.ijbiomac.2015.12.057
Statut de publication	Published - avr. 1 2016

Note bibliographique

Funding Information:
This work is supported by grant from Natural Sciences and Engineering Research Council of Canada (NSERC).

Publisher Copyright:
© 2015 Elsevier B.V.

ASJC Scopus Subject Areas

Structural Biology
Biochemistry
Molecular Biology
Economics and Econometrics
General Energy

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.ijbiomac.2015.12.057

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title = "tRNA conjugation with chitosan nanoparticles: An AFM imaging study",

abstract = "The conjugation of tRNA with chitosan nanoparticles of different sizes 15,100 and 200kDa was investigated in aqueous solution using multiple spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed that chitosan binds tRNA via G-C and A-U base pairs as well as backbone PO2 group, through electrostatic, hydrophilic and H-bonding contacts with overall binding constants of KCh-15-tRNA=4.1 (±0.60)×103M-1, KCh-100-tRNA=5.7 (±0.8)×103M-1 and KCh-200-tRNA=1.2 (±0.3)×104M-1. As chitosan size increases more stable polymer-tRNA conjugate is formed. AFM images showed major tRNA aggregation and particle formation occurred as chitosan concentration increased. Even though chitosan induced major biopolymer structural changes, tRNA remains in A-family structure.",

author = "D. Agudelo and L. Kreplak and Tajmir-Riahi, {H. A.}",

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AU - Kreplak, L.

AU - Tajmir-Riahi, H. A.

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tRNA conjugation with chitosan nanoparticles: An AFM imaging study

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Accès au document

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