Use of nitrocellulose membrane to activate and measure insect prophenol oxidase

P. T. Brey; D. B. Stoltz; D. I. Cook; M. Ashida

doi:10.1016/0003-2697(91)90241-K

Use of nitrocellulose membrane to activate and measure insect prophenol oxidase

P. T. Brey, D. B. Stoltz, D. I. Cook, M. Ashida

Medicine

Résultat de recherche: Article › examen par les pairs

7 Citations (Scopus)

Résumé

A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.

Langue d'origine	English
Pages (de-à)	359-364
Nombre de pages	6
Journal	Analytical Biochemistry
Volume	194
Numéro de publication	2
DOI	https://doi.org/10.1016/0003-2697(91)90241-K
Statut de publication	Published - mai 1 1991

Note bibliographique

Funding Information:
1 This work was supported in part Society for the Promotion of Science. ’ To whom correspondence should Biologique contre les Insectes, Institut 75724 Paris Cedex 15, France.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(91)90241-K

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title = "Use of nitrocellulose membrane to activate and measure insect prophenol oxidase",

abstract = "A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.",

author = "Brey, {P. T.} and Stoltz, {D. B.} and Cook, {D. I.} and M. Ashida",

note = "Funding Information: 1 This work was supported in part Society for the Promotion of Science. {\textquoteright} To whom correspondence should Biologique contre les Insectes, Institut 75724 Paris Cedex 15, France.",

year = "1991",

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doi = "10.1016/0003-2697(91)90241-K",

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T1 - Use of nitrocellulose membrane to activate and measure insect prophenol oxidase

AU - Brey, P. T.

AU - Stoltz, D. B.

AU - Cook, D. I.

AU - Ashida, M.

N1 - Funding Information: 1 This work was supported in part Society for the Promotion of Science. ’ To whom correspondence should Biologique contre les Insectes, Institut 75724 Paris Cedex 15, France.

PY - 1991/5/1

Y1 - 1991/5/1

N2 - A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.

AB - A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator.

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U2 - 10.1016/0003-2697(91)90241-K

DO - 10.1016/0003-2697(91)90241-K

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AN - SCOPUS:0025801188

SN - 0003-2697

VL - 194

SP - 359

EP - 364

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Use of nitrocellulose membrane to activate and measure insect prophenol oxidase

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

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