VORBEHANDLUNG INTESTINALER TRANSPLANTATE MIT 8-METHOXYPSORALEN UND UV-A-BESTRAHLUNG (PUVA-THERAPIE) - ERSTE IN-VITRO-ERGEBNISSE

Strong immunogenicity of intestinal tissue is a major problem in allogenic transplantation of small intestine. Studies were conducted into the effects of the PUVA therapy, with a view to reducing the immunogenic potential. Various psoralen concentrations (8-methoxypsoralen, 8-MOP) were used for that purpose together with several UV-A irradiation doses. Described in this paper are in vitro investigations of separated enterocytes of Wistar rat to evaluate potential side effects on intestinal mucosa, as compared to well known dose-related effects of the PUVA therapy on lymphocytes. Vitality checks were performed by means of the trypan blue test. The results so far obtained are likely to support the conclusion that in response to common combinations of 8-MOP and UV-A radiation the expected rate of damage to enterocytes should be just as high as that to lymphocytes. Therefore, a critical attitude appears to be justified to the use of PUVA therapy in connection with allogenic small intestine transplantation.