β-Glucosidase 2 (GBA2) activity and imino sugar pharmacology

Christina M. Ridley, Karen E. Thur, Jessica Shanahan, Nagendra Babu Thillaiappan, Ann Shen, Karly Uhl, Charlotte M. Walden, Ahad A. Rahim, Simon N. Waddington, Frances M. Platt, Aarnoud C. Van Der Spoel

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Background: GBA2 and GBA are both glucosidases that degrade glucosylceramide. Results: Conduritol B epoxide inactivates both GBA and GBA2, whereas the imino sugar NB-DGJ selectively inhibits GBA2. Conclusion: NB-DGJ is a suitable reagent to distinguish GBA2 from GBA. Significance: This study redefines GBA2 activity, which is relevant for clinical GBA2 measurements and imino sugar pharmacology.

Original languageEnglish
Pages (from-to)26052-26066
Number of pages15
JournalJournal of Biological Chemistry
Volume288
Issue number36
DOIs
Publication statusPublished - Sept 6 2013

ASJC Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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