TY - JOUR
T1 - β-Glucosidase 2 (GBA2) activity and imino sugar pharmacology
AU - Ridley, Christina M.
AU - Thur, Karen E.
AU - Shanahan, Jessica
AU - Thillaiappan, Nagendra Babu
AU - Shen, Ann
AU - Uhl, Karly
AU - Walden, Charlotte M.
AU - Rahim, Ahad A.
AU - Waddington, Simon N.
AU - Platt, Frances M.
AU - Van Der Spoel, Aarnoud C.
PY - 2013/9/6
Y1 - 2013/9/6
N2 - Background: GBA2 and GBA are both glucosidases that degrade glucosylceramide. Results: Conduritol B epoxide inactivates both GBA and GBA2, whereas the imino sugar NB-DGJ selectively inhibits GBA2. Conclusion: NB-DGJ is a suitable reagent to distinguish GBA2 from GBA. Significance: This study redefines GBA2 activity, which is relevant for clinical GBA2 measurements and imino sugar pharmacology.
AB - Background: GBA2 and GBA are both glucosidases that degrade glucosylceramide. Results: Conduritol B epoxide inactivates both GBA and GBA2, whereas the imino sugar NB-DGJ selectively inhibits GBA2. Conclusion: NB-DGJ is a suitable reagent to distinguish GBA2 from GBA. Significance: This study redefines GBA2 activity, which is relevant for clinical GBA2 measurements and imino sugar pharmacology.
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U2 - 10.1074/jbc.M113.463562
DO - 10.1074/jbc.M113.463562
M3 - Article
C2 - 23880767
AN - SCOPUS:84883662716
SN - 0021-9258
VL - 288
SP - 26052
EP - 26066
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 36
ER -