A factor in pollen which enhances vascular permeability

L. S. Kind; J. Banovitz; A. Menzel; B. Nilsson

doi:10.1016/0021-8707(67)90122-0

A factor in pollen which enhances vascular permeability

L. S. Kind, J. Banovitz, A. Menzel, B. Nilsson

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A method is described for the extraction and purification of a permeability-enhancing factor (PEF) from ragweed pollen. PEF is a neutral polysaccharidic compound composed of arabinose and galactose associated with small amounts of pigment and protein. As little as 35 mcg. of PEF, when injected intravenously into mice along with Evans blue dye, will induce blueing in the ears of these animals. Blueing of the ears is likely due to the release of histamine, since the administration of PEF results in the disruption of the mast cells in the ears of mice. In addition, mice injected repeatedly with compound 48/80, a material which depletes tissues of their histamine, are no longer able to respond to PEF with ear blueing. It is suggested that PEF acts by combining with natural antibody present in mouse serum or on mouse mast cells.

Original language	English
Pages (from-to)	17-24
Number of pages	8
Journal	Journal of Allergy and Clinical Immunology
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/0021-8707(67)90122-0
Publication status	Published - Jan 1967
Externally published	Yes

Bibliographical note

Funding Information:
From the Department of Asthma-A’@gy National Jewish Hospital, Denver, Cola., Roosevelt Hospital, New York, N. Y. Supported by g-rants from the National and AI-02682. Presented at the Twenty-second New York, N. Y., Feb. 22, 1966. Received for publication May 9, 196ti.

ASJC Scopus Subject Areas

General Medicine

PubMed: MeSH publication types

Journal Article

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10.1016/0021-8707(67)90122-0

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@article{03eb991158894482ab5b6940660200c4,

title = "A factor in pollen which enhances vascular permeability",

abstract = "A method is described for the extraction and purification of a permeability-enhancing factor (PEF) from ragweed pollen. PEF is a neutral polysaccharidic compound composed of arabinose and galactose associated with small amounts of pigment and protein. As little as 35 mcg. of PEF, when injected intravenously into mice along with Evans blue dye, will induce blueing in the ears of these animals. Blueing of the ears is likely due to the release of histamine, since the administration of PEF results in the disruption of the mast cells in the ears of mice. In addition, mice injected repeatedly with compound 48/80, a material which depletes tissues of their histamine, are no longer able to respond to PEF with ear blueing. It is suggested that PEF acts by combining with natural antibody present in mouse serum or on mouse mast cells.",

author = "Kind, {L. S.} and J. Banovitz and A. Menzel and B. Nilsson",

note = "Funding Information: From the Department of Asthma-A{\textquoteright}@gy National Jewish Hospital, Denver, Cola., Roosevelt Hospital, New York, N. Y. Supported by g-rants from the National and AI-02682. Presented at the Twenty-second New York, N. Y., Feb. 22, 1966. Received for publication May 9, 196ti.",

year = "1967",

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doi = "10.1016/0021-8707(67)90122-0",

language = "English",

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journal = "Journal of Allergy and Clinical Immunology",

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TY - JOUR

T1 - A factor in pollen which enhances vascular permeability

AU - Kind, L. S.

AU - Banovitz, J.

AU - Menzel, A.

AU - Nilsson, B.

N1 - Funding Information: From the Department of Asthma-A’@gy National Jewish Hospital, Denver, Cola., Roosevelt Hospital, New York, N. Y. Supported by g-rants from the National and AI-02682. Presented at the Twenty-second New York, N. Y., Feb. 22, 1966. Received for publication May 9, 196ti.

PY - 1967/1

Y1 - 1967/1

N2 - A method is described for the extraction and purification of a permeability-enhancing factor (PEF) from ragweed pollen. PEF is a neutral polysaccharidic compound composed of arabinose and galactose associated with small amounts of pigment and protein. As little as 35 mcg. of PEF, when injected intravenously into mice along with Evans blue dye, will induce blueing in the ears of these animals. Blueing of the ears is likely due to the release of histamine, since the administration of PEF results in the disruption of the mast cells in the ears of mice. In addition, mice injected repeatedly with compound 48/80, a material which depletes tissues of their histamine, are no longer able to respond to PEF with ear blueing. It is suggested that PEF acts by combining with natural antibody present in mouse serum or on mouse mast cells.

AB - A method is described for the extraction and purification of a permeability-enhancing factor (PEF) from ragweed pollen. PEF is a neutral polysaccharidic compound composed of arabinose and galactose associated with small amounts of pigment and protein. As little as 35 mcg. of PEF, when injected intravenously into mice along with Evans blue dye, will induce blueing in the ears of these animals. Blueing of the ears is likely due to the release of histamine, since the administration of PEF results in the disruption of the mast cells in the ears of mice. In addition, mice injected repeatedly with compound 48/80, a material which depletes tissues of their histamine, are no longer able to respond to PEF with ear blueing. It is suggested that PEF acts by combining with natural antibody present in mouse serum or on mouse mast cells.

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AN - SCOPUS:0014037978

SN - 0021-8707

VL - 39

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JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 1

ER -

A factor in pollen which enhances vascular permeability

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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