Aortic valve grafts in the rat: Evidence for rejection

A. Moustapha; D. B. Ross; B. Bittira; D. Van-Velzen; V. C. McAlister; C. L. Lannon; T. D. Lee; D. A. Murphy; C. A. Yankah; M. Yacoub

doi:10.1016/S0022-5223(97)70002-3

Aortic valve grafts in the rat: Evidence for rejection

A. Moustapha, D. B. Ross, B. Bittira, D. Van-Velzen, V. C. McAlister, C. L. Lannon, T. D. Lee, D. A. Murphy, C. A. Yankah, M. Yacoub

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Objective: The histopathologic changes of fresh rat aortic valve allografts over time and the effect of cryopreservation were examined. Methods: Fifty-six syngeneic and allogeneic rat aortic valves were transplanted, either fresh or after cryopreservation, and then at different time points they were explanted and histologically examined in a blinded fashion. Results: Histopathologic changes in the first week are similar in syngeneic and allogeneic grafts. Fresh syngeneic grafts and leaflets retained normal structure up to 56 days. Allogeneic grafts showed retrovalvular thrombus formation with leaflet ghosts and neointimal proliferation. Cryopreservation did not alter this process. Conclusions: Cardiac allograft valves in the rat model undergo changes that are characteristic of cell- mediated rejection and lead to valve failure.

Original language	English
Pages (from-to)	891-902
Number of pages	12
Journal	Journal of Thoracic and Cardiovascular Surgery
Volume	114
Issue number	6
DOIs	https://doi.org/10.1016/S0022-5223(97)70002-3
Publication status	Published - 1997
Externally published	Yes

Bibliographical note

Funding Information:
We gratefully acknowledge the financial support of the Izaak-Walton-Killam Hospital for Children to this study. We thank Mr. Lewis Page, Mr. André Le Prairie, and members of the Regional Tissue Bank, Queen Elizabeth II Health Sciences Centre, Halifax, Canada, for their assistance with the cryopreservation. In addition we would like to thank Mrs. Mary Wile for her expertise with the histologic preparation.

ASJC Scopus Subject Areas

Surgery
Pulmonary and Respiratory Medicine
Cardiology and Cardiovascular Medicine

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/S0022-5223(97)70002-3

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title = "Aortic valve grafts in the rat: Evidence for rejection",

abstract = "Objective: The histopathologic changes of fresh rat aortic valve allografts over time and the effect of cryopreservation were examined. Methods: Fifty-six syngeneic and allogeneic rat aortic valves were transplanted, either fresh or after cryopreservation, and then at different time points they were explanted and histologically examined in a blinded fashion. Results: Histopathologic changes in the first week are similar in syngeneic and allogeneic grafts. Fresh syngeneic grafts and leaflets retained normal structure up to 56 days. Allogeneic grafts showed retrovalvular thrombus formation with leaflet ghosts and neointimal proliferation. Cryopreservation did not alter this process. Conclusions: Cardiac allograft valves in the rat model undergo changes that are characteristic of cell- mediated rejection and lead to valve failure.",

author = "A. Moustapha and Ross, {D. B.} and B. Bittira and D. Van-Velzen and McAlister, {V. C.} and Lannon, {C. L.} and Lee, {T. D.} and Murphy, {D. A.} and Yankah, {C. A.} and M. Yacoub",

note = "Funding Information: We gratefully acknowledge the financial support of the Izaak-Walton-Killam Hospital for Children to this study. We thank Mr. Lewis Page, Mr. Andr{\'e} Le Prairie, and members of the Regional Tissue Bank, Queen Elizabeth II Health Sciences Centre, Halifax, Canada, for their assistance with the cryopreservation. In addition we would like to thank Mrs. Mary Wile for her expertise with the histologic preparation. ",

year = "1997",

doi = "10.1016/S0022-5223(97)70002-3",

language = "English",

volume = "114",

pages = "891--902",

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TY - JOUR

T1 - Aortic valve grafts in the rat

T2 - Evidence for rejection

AU - Moustapha, A.

AU - Ross, D. B.

AU - Bittira, B.

AU - Van-Velzen, D.

AU - McAlister, V. C.

AU - Lannon, C. L.

AU - Lee, T. D.

AU - Murphy, D. A.

AU - Yankah, C. A.

AU - Yacoub, M.

N1 - Funding Information: We gratefully acknowledge the financial support of the Izaak-Walton-Killam Hospital for Children to this study. We thank Mr. Lewis Page, Mr. André Le Prairie, and members of the Regional Tissue Bank, Queen Elizabeth II Health Sciences Centre, Halifax, Canada, for their assistance with the cryopreservation. In addition we would like to thank Mrs. Mary Wile for her expertise with the histologic preparation.

PY - 1997

Y1 - 1997

N2 - Objective: The histopathologic changes of fresh rat aortic valve allografts over time and the effect of cryopreservation were examined. Methods: Fifty-six syngeneic and allogeneic rat aortic valves were transplanted, either fresh or after cryopreservation, and then at different time points they were explanted and histologically examined in a blinded fashion. Results: Histopathologic changes in the first week are similar in syngeneic and allogeneic grafts. Fresh syngeneic grafts and leaflets retained normal structure up to 56 days. Allogeneic grafts showed retrovalvular thrombus formation with leaflet ghosts and neointimal proliferation. Cryopreservation did not alter this process. Conclusions: Cardiac allograft valves in the rat model undergo changes that are characteristic of cell- mediated rejection and lead to valve failure.

AB - Objective: The histopathologic changes of fresh rat aortic valve allografts over time and the effect of cryopreservation were examined. Methods: Fifty-six syngeneic and allogeneic rat aortic valves were transplanted, either fresh or after cryopreservation, and then at different time points they were explanted and histologically examined in a blinded fashion. Results: Histopathologic changes in the first week are similar in syngeneic and allogeneic grafts. Fresh syngeneic grafts and leaflets retained normal structure up to 56 days. Allogeneic grafts showed retrovalvular thrombus formation with leaflet ghosts and neointimal proliferation. Cryopreservation did not alter this process. Conclusions: Cardiac allograft valves in the rat model undergo changes that are characteristic of cell- mediated rejection and lead to valve failure.

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Aortic valve grafts in the rat: Evidence for rejection

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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