Class II MHC expression on rat intestinal intraepithelial lymphocytes

Joy A. Kearsey; Andrew W. Stadnyk

doi:10.1016/S0165-2478(96)02684-3

Class II MHC expression on rat intestinal intraepithelial lymphocytes

Joy A. Kearsey, Andrew W. Stadnyk

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The expression of class II major histocompatibility complex (MHC) on rat intestinal intraepithelial lymphocytes (IELs) and mesenteric lymph node cells (MLNCs) were examined by immunofluorescence and flow cytometry. As expected, MLNCs contain eight small populations of CD4⁺ class II MHC⁺ T-cells in addition to classical antigen presenting cells. In contrast, rat IELs include a significant population of class II MHC⁺ T-cells, predominantly in the CD8⁺ CD4^- αβTCR⁺ subset. IEL samples with a relatively high percentage of class II MHC⁺ cells also include some CD4⁺ class II MHC⁺ cells; IEL samples with a low percentage of class II MHC⁺ cells also include some CD4^- CD8^- class II MHC⁺ cells. The role of lymphocyte subpopulations in the intestinal epithelium may need to be revisited in consideration of these findings.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	Immunology Letters
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/S0165-2478(96)02684-3
Publication status	Published - Feb 1997

Bibliographical note

Funding Information:
This study was supported by the Crohn’s and Colitis Foundation of Canada, and the Natural Sciences and Engineering Research Council of Canada. Dr Stadnyk is the recipient of an IWK Children’s Hospital Research Investigatorship. The authors thank Dr Linda Best for assistance with the flow cytometry.

ASJC Scopus Subject Areas

Immunology and Allergy
Immunology

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@article{aca679a727ed4f2ba338b3f803fca621,

title = "Class II MHC expression on rat intestinal intraepithelial lymphocytes",

abstract = "The expression of class II major histocompatibility complex (MHC) on rat intestinal intraepithelial lymphocytes (IELs) and mesenteric lymph node cells (MLNCs) were examined by immunofluorescence and flow cytometry. As expected, MLNCs contain eight small populations of CD4+ class II MHC+ T-cells in addition to classical antigen presenting cells. In contrast, rat IELs include a significant population of class II MHC+ T-cells, predominantly in the CD8+ CD4- αβTCR+ subset. IEL samples with a relatively high percentage of class II MHC+ cells also include some CD4+ class II MHC+ cells; IEL samples with a low percentage of class II MHC+ cells also include some CD4- CD8- class II MHC+ cells. The role of lymphocyte subpopulations in the intestinal epithelium may need to be revisited in consideration of these findings.",

author = "Kearsey, {Joy A.} and Stadnyk, {Andrew W.}",

note = "Funding Information: This study was supported by the Crohn{\textquoteright}s and Colitis Foundation of Canada, and the Natural Sciences and Engineering Research Council of Canada. Dr Stadnyk is the recipient of an IWK Children{\textquoteright}s Hospital Research Investigatorship. The authors thank Dr Linda Best for assistance with the flow cytometry.",

year = "1997",

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T1 - Class II MHC expression on rat intestinal intraepithelial lymphocytes

AU - Kearsey, Joy A.

AU - Stadnyk, Andrew W.

N1 - Funding Information: This study was supported by the Crohn’s and Colitis Foundation of Canada, and the Natural Sciences and Engineering Research Council of Canada. Dr Stadnyk is the recipient of an IWK Children’s Hospital Research Investigatorship. The authors thank Dr Linda Best for assistance with the flow cytometry.

PY - 1997/2

Y1 - 1997/2

N2 - The expression of class II major histocompatibility complex (MHC) on rat intestinal intraepithelial lymphocytes (IELs) and mesenteric lymph node cells (MLNCs) were examined by immunofluorescence and flow cytometry. As expected, MLNCs contain eight small populations of CD4+ class II MHC+ T-cells in addition to classical antigen presenting cells. In contrast, rat IELs include a significant population of class II MHC+ T-cells, predominantly in the CD8+ CD4- αβTCR+ subset. IEL samples with a relatively high percentage of class II MHC+ cells also include some CD4+ class II MHC+ cells; IEL samples with a low percentage of class II MHC+ cells also include some CD4- CD8- class II MHC+ cells. The role of lymphocyte subpopulations in the intestinal epithelium may need to be revisited in consideration of these findings.

AB - The expression of class II major histocompatibility complex (MHC) on rat intestinal intraepithelial lymphocytes (IELs) and mesenteric lymph node cells (MLNCs) were examined by immunofluorescence and flow cytometry. As expected, MLNCs contain eight small populations of CD4+ class II MHC+ T-cells in addition to classical antigen presenting cells. In contrast, rat IELs include a significant population of class II MHC+ T-cells, predominantly in the CD8+ CD4- αβTCR+ subset. IEL samples with a relatively high percentage of class II MHC+ cells also include some CD4+ class II MHC+ cells; IEL samples with a low percentage of class II MHC+ cells also include some CD4- CD8- class II MHC+ cells. The role of lymphocyte subpopulations in the intestinal epithelium may need to be revisited in consideration of these findings.

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DO - 10.1016/S0165-2478(96)02684-3

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SN - 0165-2478

VL - 55

SP - 63

EP - 68

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

Class II MHC expression on rat intestinal intraepithelial lymphocytes

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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