Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Rimas Nivinskas; Rita Vaiškunaite; Ramune Dagyte; Aušra Raudonikiene; Vytautas Klausa

doi:10.1016/0042-6822(92)90547-3

Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Rimas Nivinskas, Rita Vaiškunaite, Ramune Dagyte, Aušra Raudonikiene, Vytautas Klausa

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an M_r of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

Original language	English
Pages (from-to)	887-889
Number of pages	3
Journal	Virology
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(92)90547-3
Publication status	Published - Jun 1992
Externally published	Yes

ASJC Scopus Subject Areas

Virology

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10.1016/0042-6822(92)90547-3

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title = "Cloning, sequence, and overexpression of bacteriophage T4 gene 51",

abstract = "The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.",

author = "Rimas Nivinskas and Rita Vai{\v s}kunaite and Ramune Dagyte and Au{\v s}ra Raudonikiene and Vytautas Klausa",

year = "1992",

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T1 - Cloning, sequence, and overexpression of bacteriophage T4 gene 51

AU - Nivinskas, Rimas

AU - Vaiškunaite, Rita

AU - Dagyte, Ramune

AU - Raudonikiene, Aušra

AU - Klausa, Vytautas

PY - 1992/6

Y1 - 1992/6

N2 - The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

AB - The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

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Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Abstract

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