Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Rimas Nivinskas; Rita Vaiškunaite; Ramune Dagyte; Aušra Raudonikiene; Vytautas Klausa

doi:10.1016/0042-6822(92)90547-3

Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Rimas Nivinskas, Rita Vaiškunaite, Ramune Dagyte, Aušra Raudonikiene, Vytautas Klausa

Résultat de recherche: Article › examen par les pairs

4 Citations (Scopus)

Résumé

The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an M_r of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

Langue d'origine	English
Pages (de-à)	887-889
Nombre de pages	3
Journal	Virology
Volume	188
Numéro de publication	2
DOI	https://doi.org/10.1016/0042-6822(92)90547-3
Statut de publication	Published - juin 1992
Publié à l'externe	Oui

ASJC Scopus Subject Areas

Virology

Accès au document

10.1016/0042-6822(92)90547-3

Autres fichiers et liens

Citer

@article{edc2c81c01d241b094a393927127fcd5,

title = "Cloning, sequence, and overexpression of bacteriophage T4 gene 51",

abstract = "The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.",

author = "Rimas Nivinskas and Rita Vai{\v s}kunaite and Ramune Dagyte and Au{\v s}ra Raudonikiene and Vytautas Klausa",

year = "1992",

month = jun,

doi = "10.1016/0042-6822(92)90547-3",

language = "English",

volume = "188",

pages = "887--889",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Cloning, sequence, and overexpression of bacteriophage T4 gene 51

AU - Nivinskas, Rimas

AU - Vaiškunaite, Rita

AU - Dagyte, Ramune

AU - Raudonikiene, Aušra

AU - Klausa, Vytautas

PY - 1992/6

Y1 - 1992/6

N2 - The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

AB - The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an Mr of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also show that the low level of gene 51 expression usually seen is caused by an RNA stem-loop structure in the region between genes 26 and 51. In addition, discrepancies with the sequence published by other authors are indicated.

UR - http://www.scopus.com/inward/record.url?scp=0026708735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026708735&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(92)90547-3

DO - 10.1016/0042-6822(92)90547-3

M3 - Article

C2 - 1585655

AN - SCOPUS:0026708735

SN - 0042-6822

VL - 188

SP - 887

EP - 889

JO - Virology

JF - Virology

IS - 2

ER -

Cloning, sequence, and overexpression of bacteriophage T4 gene 51

Résumé

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

Citer