Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

John P. Frampton; Huilin Shi; Albert Kao; Jack M. Parent; Shuichi Takayama

doi:10.1002/adhm.201300049

Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

John P. Frampton, Huilin Shi, Albert Kao, Jack M. Parent, Shuichi Takayama

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.

Original language	English
Pages (from-to)	1440-1444
Number of pages	5
Journal	Advanced healthcare materials
Volume	2
Issue number	11
DOIs	https://doi.org/10.1002/adhm.201300049
Publication status	Published - Nov 2013
Externally published	Yes

ASJC Scopus Subject Areas

Biomaterials
Biomedical Engineering
Pharmaceutical Science

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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10.1002/adhm.201300049

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Frampton, J. P., Shi, H., Kao, A., Parent, J. M., & Takayama, S. (2013). Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes. Advanced healthcare materials, 2(11), 1440-1444. https://doi.org/10.1002/adhm.201300049

Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes. / Frampton, John P.; Shi, Huilin; Kao, Albert et al.
In: Advanced healthcare materials, Vol. 2, No. 11, 11.2013, p. 1440-1444.

Research output: Contribution to journal › Article › peer-review

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Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

Abstract

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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