Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

John P. Frampton; Huilin Shi; Albert Kao; Jack M. Parent; Shuichi Takayama

doi:10.1002/adhm.201300049

Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

John P. Frampton, Huilin Shi, Albert Kao, Jack M. Parent, Shuichi Takayama

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11 Citas (Scopus)

Resumen

Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.

Idioma original	English
Páginas (desde-hasta)	1440-1444
Número de páginas	5
Publicación	Advanced healthcare materials
Volumen	2
N.º	11
DOI	https://doi.org/10.1002/adhm.201300049
Estado	Published - nov. 2013
Publicado de forma externa	Sí

ASJC Scopus Subject Areas

Biomaterials
Biomedical Engineering
Pharmaceutical Science

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes. / Frampton, John P.; Shi, Huilin; Kao, Albert et al.
En: Advanced healthcare materials, Vol. 2, N.º 11, 11.2013, p. 1440-1444.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

Resumen

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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