Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

Marvin Gold; Christopher W. Helleiner; Maire Percy

doi:10.1016/0926-6550(64)90092-1

Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

Marvin Gold, Christopher W. Helleiner, Maire Percy

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.

Original language	English
Pages (from-to)	204-212
Number of pages	9
Journal	BBA Specialized Section on Nucleic Acids and Related Subjects
Volume	80
Issue number	2
DOIs	https://doi.org/10.1016/0926-6550(64)90092-1
Publication status	Published - Feb 17 1964
Externally published	Yes

Bibliographical note

Funding Information:
We wish to express our gratitude to Dr. L. SIMINOVlTCH for his interest in, and helpful comments on this work, and to Mrs. I. Oss for technical assistance. Financial support was received from the National Cancer Institute of Canada and the Medical Research Council of Canada. Hoffmann-La Roche of Canada, Ltd., kindly supplied the FdeUrd used. One of us (M.G.) held a W. P. Caven Memorial Fellowship, and another (M.P.) a National Research Council of Canada Bursary.

ASJC Scopus Subject Areas

General Medicine

PubMed: MeSH publication types

Journal Article

Access to Document

10.1016/0926-6550(64)90092-1

Cite this

@article{0da83944839b488a892e1754c3e668d9,

title = "Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays",

abstract = "1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.",

author = "Marvin Gold and Helleiner, {Christopher W.} and Maire Percy",

note = "Funding Information: We wish to express our gratitude to Dr. L. SIMINOVlTCH for his interest in, and helpful comments on this work, and to Mrs. I. Oss for technical assistance. Financial support was received from the National Cancer Institute of Canada and the Medical Research Council of Canada. Hoffmann-La Roche of Canada, Ltd., kindly supplied the FdeUrd used. One of us (M.G.) held a W. P. Caven Memorial Fellowship, and another (M.P.) a National Research Council of Canada Bursary.",

year = "1964",

month = feb,

day = "17",

doi = "10.1016/0926-6550(64)90092-1",

language = "English",

volume = "80",

pages = "204--212",

journal = "BBA Specialized Section on Nucleic Acids and Related Subjects",

issn = "0926-6550",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

AU - Gold, Marvin

AU - Helleiner, Christopher W.

AU - Percy, Maire

N1 - Funding Information: We wish to express our gratitude to Dr. L. SIMINOVlTCH for his interest in, and helpful comments on this work, and to Mrs. I. Oss for technical assistance. Financial support was received from the National Cancer Institute of Canada and the Medical Research Council of Canada. Hoffmann-La Roche of Canada, Ltd., kindly supplied the FdeUrd used. One of us (M.G.) held a W. P. Caven Memorial Fellowship, and another (M.P.) a National Research Council of Canada Bursary.

PY - 1964/2/17

Y1 - 1964/2/17

N2 - 1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.

AB - 1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.

UR - http://www.scopus.com/inward/record.url?scp=50549212049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50549212049&partnerID=8YFLogxK

U2 - 10.1016/0926-6550(64)90092-1

DO - 10.1016/0926-6550(64)90092-1

M3 - Article

C2 - 14164205

AN - SCOPUS:50549212049

SN - 0926-6550

VL - 80

SP - 204

EP - 212

JO - BBA Specialized Section on Nucleic Acids and Related Subjects

JF - BBA Specialized Section on Nucleic Acids and Related Subjects

IS - 2

ER -

Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

Other files and links

Fingerprint

Cite this