Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

Marvin Gold; Christopher W. Helleiner; Maire Percy

doi:10.1016/0926-6550(64)90092-1

Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

Marvin Gold, Christopher W. Helleiner, Maire Percy

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.

Idioma original	English
Páginas (desde-hasta)	204-212
Número de páginas	9
Publicación	BBA Specialized Section on Nucleic Acids and Related Subjects
Volumen	80
N.º	2
DOI	https://doi.org/10.1016/0926-6550(64)90092-1
Estado	Published - feb. 17 1964
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
We wish to express our gratitude to Dr. L. SIMINOVlTCH for his interest in, and helpful comments on this work, and to Mrs. I. Oss for technical assistance. Financial support was received from the National Cancer Institute of Canada and the Medical Research Council of Canada. Hoffmann-La Roche of Canada, Ltd., kindly supplied the FdeUrd used. One of us (M.G.) held a W. P. Caven Memorial Fellowship, and another (M.P.) a National Research Council of Canada Bursary.

ASJC Scopus Subject Areas

General Medicine

PubMed: MeSH publication types

Journal Article

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Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays. / Gold, Marvin; Helleiner, Christopher W.; Percy, Maire.
En: BBA Specialized Section on Nucleic Acids and Related Subjects, Vol. 80, N.º 2, 17.02.1964, p. 204-212.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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abstract = "1. 1. When randomly-growing cultures of L cells are irradiated with 1000-3000 rads of X-rays, DNA synthesis continues for a time, stops briefly, and is then resumed. The timing of these events depends on the dose of X-rays. 2. 2. The DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyl-transferase, EC 2.7.7.7) activity of extracts made from cells irradiated in this way reaches a minimum when DNA synthesis is stopped. 3. 3. When synchronized cultures of L-cells are irradiated with X-rays, DNA synthesis and cell division are delayed. 4. 4. The decrease in the activity of DNA polymerase normally seen in extracts of cells engaged in active DNA synthesis is also delayed by the irradiation. These effects are dependent on the dose and also on the time when the dose is administered.",

author = "Marvin Gold and Helleiner, {Christopher W.} and Maire Percy",

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Deoxyribonucleic acid polymerase in L cells. II. Deoxyribonucleic acid polymerase activity in extracts of cells treated with x-rays

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PubMed: MeSH publication types

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