Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

Jason J. Leblanc, Janice Pettipas, Ross J. Davidson, Graham A. Tipples, Joanne Hiebert, Todd F. Hatchette

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.

Original languageEnglish
Pages (from-to)4049-4051
Number of pages3
JournalJournal of Clinical Microbiology
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 2008

ASJC Scopus Subject Areas

  • Microbiology (medical)

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