Abstract
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
| Original language | English |
|---|---|
| Pages (from-to) | 4049-4051 |
| Number of pages | 3 |
| Journal | Journal of Clinical Microbiology |
| Volume | 46 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2008 |
ASJC Scopus Subject Areas
- Microbiology (medical)
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Leblanc, J. J., Pettipas, J., Davidson, R. J., Tipples, G. A., Hiebert, J., & Hatchette, T. F. (2008). Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform. Journal of Clinical Microbiology, 46(12), 4049-4051. https://doi.org/10.1128/JCM.01446-08