Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

Jason J. Leblanc; Janice Pettipas; Ross J. Davidson; Graham A. Tipples; Joanne Hiebert; Todd F. Hatchette

doi:10.1128/JCM.01446-08

Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

Jason J. Leblanc, Janice Pettipas, Ross J. Davidson, Graham A. Tipples, Joanne Hiebert, Todd F. Hatchette

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

10 Citations (Scopus)

Résumé

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.

Langue d'origine	English
Pages (de-à)	4049-4051
Nombre de pages	3
Journal	Journal of Clinical Microbiology
Volume	46
Numéro de publication	12
DOI	https://doi.org/10.1128/JCM.01446-08
Statut de publication	Published - déc. 2008

ASJC Scopus Subject Areas

Microbiology (medical)

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10.1128/JCM.01446-08

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abstract = "A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.",

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AU - Hiebert, Joanne

AU - Hatchette, Todd F.

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Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

Résumé

ASJC Scopus Subject Areas

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