Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

D. Roy; I. G. Paterson; T. R. Hurlbut; D. E. Ruzzante

doi:10.1007/s12686-009-9140-6

Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

D. Roy, I. G. Paterson, T. R. Hurlbut, D. E. Ruzzante

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.

Original language	English
Pages (from-to)	45-49
Number of pages	5
Journal	Conservation Genetics Resources
Volume	2
Issue number	1
DOIs	https://doi.org/10.1007/s12686-009-9140-6
Publication status	Published - Dec 2010

Bibliographical note

Funding Information:
Acknowledgments This work was supported by Department of Fisheries and Oceans grants to DER & TRH and by a Natural Sciences and Engineering Council of Canada Discovery Grant to DER. The authors would like to thank Laurent Kreplak of the Dept. of Physics & Atmospheric Sciences at Dalhousie for equipment loans.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

Access to Document

10.1007/s12686-009-9140-6

Cite this

@article{609b275ad1be4dfcbc373baab192f599,

title = "Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic",

abstract = "We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.",

author = "D. Roy and Paterson, {I. G.} and Hurlbut, {T. R.} and Ruzzante, {D. E.}",

note = "Funding Information: Acknowledgments This work was supported by Department of Fisheries and Oceans grants to DER & TRH and by a Natural Sciences and Engineering Council of Canada Discovery Grant to DER. The authors would like to thank Laurent Kreplak of the Dept. of Physics & Atmospheric Sciences at Dalhousie for equipment loans.",

year = "2010",

month = dec,

doi = "10.1007/s12686-009-9140-6",

language = "English",

volume = "2",

pages = "45--49",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

AU - Roy, D.

AU - Paterson, I. G.

AU - Hurlbut, T. R.

AU - Ruzzante, D. E.

N1 - Funding Information: Acknowledgments This work was supported by Department of Fisheries and Oceans grants to DER & TRH and by a Natural Sciences and Engineering Council of Canada Discovery Grant to DER. The authors would like to thank Laurent Kreplak of the Dept. of Physics & Atmospheric Sciences at Dalhousie for equipment loans.

PY - 2010/12

Y1 - 2010/12

N2 - We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.

AB - We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.

UR - http://www.scopus.com/inward/record.url?scp=84857828496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857828496&partnerID=8YFLogxK

U2 - 10.1007/s12686-009-9140-6

DO - 10.1007/s12686-009-9140-6

M3 - Article

AN - SCOPUS:84857828496

SN - 1877-7252

VL - 2

SP - 45

EP - 49

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 1

ER -

Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Access to Document

Other files and links

Fingerprint

Cite this