Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

D. Roy; I. G. Paterson; T. R. Hurlbut; D. E. Ruzzante

doi:10.1007/s12686-009-9140-6

Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

D. Roy, I. G. Paterson, T. R. Hurlbut, D. E. Ruzzante

Medicine

Medicine

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2 Citas (Scopus)

Resumen

We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.

Idioma original	English
Páginas (desde-hasta)	45-49
Número de páginas	5
Publicación	Conservation Genetics Resources
Volumen	2
N.º	1
DOI	https://doi.org/10.1007/s12686-009-9140-6
Estado	Published - dic. 2010

Nota bibliográfica

Funding Information:
Acknowledgments This work was supported by Department of Fisheries and Oceans grants to DER & TRH and by a Natural Sciences and Engineering Council of Canada Discovery Grant to DER. The authors would like to thank Laurent Kreplak of the Dept. of Physics & Atmospheric Sciences at Dalhousie for equipment loans.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

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Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic. / Roy, D.; Paterson, I. G.; Hurlbut, T. R. et al.
En: Conservation Genetics Resources, Vol. 2, N.º 1, 12.2010, p. 45-49.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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abstract = "We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.",

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AU - Ruzzante, D. E.

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Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

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Nota bibliográfica

ASJC Scopus Subject Areas

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