Enolase is present at the centrosome of HeLa cells

Sharon A. Johnstone; David Morton Waisman; J. B. Rattner

doi:10.1016/0014-4827(92)90099-T

Enolase is present at the centrosome of HeLa cells

Sharon A. Johnstone, David Morton Waisman, J. B. Rattner

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

Original language	English
Pages (from-to)	458-463
Number of pages	6
Journal	Experimental Cell Research
Volume	202
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(92)90099-T
Publication status	Published - Oct 1992
Externally published	Yes

Bibliographical note

Funding Information:
This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.

ASJC Scopus Subject Areas

Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0014-4827(92)90099-T

Cite this

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title = "Enolase is present at the centrosome of HeLa cells",

abstract = "Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.",

author = "Johnstone, {Sharon A.} and Waisman, {David Morton} and Rattner, {J. B.}",

note = "Funding Information: This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.",

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doi = "10.1016/0014-4827(92)90099-T",

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AU - Johnstone, Sharon A.

AU - Waisman, David Morton

AU - Rattner, J. B.

N1 - Funding Information: This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.

PY - 1992/10

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N2 - Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

AB - Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

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Enolase is present at the centrosome of HeLa cells

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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