Enolase is present at the centrosome of HeLa cells

Sharon A. Johnstone; David Morton Waisman; J. B. Rattner

doi:10.1016/0014-4827(92)90099-T

Enolase is present at the centrosome of HeLa cells

Sharon A. Johnstone, David Morton Waisman, J. B. Rattner

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Resumen

Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

Idioma original	English
Páginas (desde-hasta)	458-463
Número de páginas	6
Publicación	Experimental Cell Research
Volumen	202
N.º	2
DOI	https://doi.org/10.1016/0014-4827(92)90099-T
Estado	Published - oct. 1992
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.

ASJC Scopus Subject Areas

Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0014-4827(92)90099-T

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title = "Enolase is present at the centrosome of HeLa cells",

abstract = "Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.",

author = "Johnstone, {Sharon A.} and Waisman, {David Morton} and Rattner, {J. B.}",

note = "Funding Information: This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.",

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doi = "10.1016/0014-4827(92)90099-T",

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T1 - Enolase is present at the centrosome of HeLa cells

AU - Johnstone, Sharon A.

AU - Waisman, David Morton

AU - Rattner, J. B.

N1 - Funding Information: This work is supported by a grant from the Medical Research Council of Canada (D.M.W.) and the National Cancer Institute of Canada (J.B.R.). The authors thank Craig Litwin for help in preparing the centrosomal preparations and Jerry Wang for his helpful comments during the course of this investigation and manuscript preparation.

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N2 - Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

AB - Antibodies raised against the C-terminus and N-terminus region of γγ enolase, as well as a polyclonal antibody raised against bovine brain γγ enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.

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Enolase is present at the centrosome of HeLa cells

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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